Literature summary for 3.1.1.25 extracted from

  • Le, Q.A.; Kim, S.; Chang, R.; Kim, Y.H.
    Insights into the lactonase mechanism of serum paraoxonase 1 (PON1) experimental and quantum mechanics/molecular mechanics (QM/MM) studies (2015), J. Phys. Chem. B, 119, 9571-9585 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami B (DE3) cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.316
-
(R)-gamma-valerolactone at pH 7.0 and 30°C Homo sapiens
36.8
-
gamma-butyrolactone at pH 7.0 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 4.5 mM used in assay conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gamma-butyrolactone + H2O
-
Homo sapiens 4-hydroxybutanoate
-
?
(R)-gamma-valerolactone + H2O
-
Homo sapiens 4-hydroxypentanoic acid
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
147.209
-
gamma-butyrolactone at pH 7.0 and 30°C Homo sapiens
36.621
-
(R)-gamma-valerolactone at pH 7.0 and 30°C Homo sapiens

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4
-
gamma-butyrolactone at pH 7.0 and 30°C Homo sapiens
27.82
-
(R)-gamma-valerolactone at pH 7.0 and 30°C Homo sapiens