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Literature summary for 3.1.1.25 extracted from
- Characterization of a phosphotriesterase-like lactonase from the hyperthermoacidophilic crenarchaeon Vulcanisaeta moutnovskia (2014), J. Biotechnol., 190, 11-17.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli | Vulcanisaeta moutnovskia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.05-10 mM, nearly complete loss of activity | Vulcanisaeta moutnovskia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.75 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 0.93 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 1.95 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 4.56 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 11.57 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
| Co2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
| Mn2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
| Ni2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
| Zn2+ | the catalytic activity strictly depended on bivalent cations (Cd2+> Ni2+> Co2+> Mn2+> Zn2+) | Vulcanisaeta moutnovskia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | Vulcanisaeta moutnovskia |
| 82000 | - |
gel filtration | Vulcanisaeta moutnovskia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vulcanisaeta moutnovskia | F0QXN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vulcanisaeta moutnovskia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-butyl-4-methyldihydro-2(3H)-furanone + H2O | i.e. whiskey lactone | Vulcanisaeta moutnovskia | 4-hydroxy-3-methyl-octanoate | - |
? | |
| 9-oxo-N-(2-oxotetrahydrofuran-3-yl)undecanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-[(9-oxoundecanoyl)amino]propanoate | - |
? | |
| gamma-(R)-caprolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxyhexanoate | - |
? | |
| gamma-(R)-valerolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxypentanoate | - |
? | |
| gamma-(S)-caprolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxyhexanoate | - |
? | |
| gamma-(S)-valerolactone + H2O | slight preference but not an exclusive specificity for the (R)-enantiomers of capro- and valerolactone | Vulcanisaeta moutnovskia | 4-hydroxypentanoate | - |
? | |
| gamma-butyrolactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxybutanoate | - |
? | |
| N-(2-oxotetrahydrofuran-3-yl)nonanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-(nonanoylamino)propanoate | - |
? | |
| N-(2-oxotetrahydrofuran-3-yl)pentanamide + H2O | - |
Vulcanisaeta moutnovskia | 3-hydroxy-2-(pentanoylamino)propanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 36000, SDS-PAGE | Vulcanisaeta moutnovskia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Vmut2255 | - |
Vulcanisaeta moutnovskia |
| VmutPLL | - |
Vulcanisaeta moutnovskia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | 70°C: about 60% of maximal activity, 90°C: about 50% of maximal activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
3 h, 30% loss of specific activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
| 80 | - |
half-life: 2 h, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
| 90 | - |
half-life: 26 min, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.89 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 2.68 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 2.79 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.04 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 4.2 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 6.23 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 30% of maximal activity, pH 9.0: about 80% of maximal activity, substrate: methyl paraoxon | Vulcanisaeta moutnovskia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
gamma-butyrolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 1.33 | - |
gamma-(R)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 1.38 | - |
gamma-(S)-valerolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 2.53 | - |
gamma-(S)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 4.54 | - |
5-butyl-4-methyldihydro-2(3H)-furanone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 5.56 | - |
gamma-(R)-caprolactone | pH 8.3, temperature not specified in the publication | Vulcanisaeta moutnovskia | |
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