Literature summary for 3.1.1.25 extracted from

  • Kataoka, M.; Nomura, J.I.; Shinohara, M.; Nose, K.; Sakamoto, K.; Shimizu, S.
    Purification and characterization of a novel lactonohydrolase from Agrobacterium tumefaciens (2000), Biosci. Biotechnol. Biochem., 64, 1255-1262.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline at 2 mM, 50% residual activity Agrobacterium tumefaciens
AlCl3 at 2 mM, 66% residual activity Agrobacterium tumefaciens
EDTA at 2 mM, 85% residual activity Agrobacterium tumefaciens
FeCl2 at 2 mM, 69% residual activity Agrobacterium tumefaciens
FeCl3 at 2 mM, 43% residual activity Agrobacterium tumefaciens
NaN3 at 2 mM, 84% residual activity Agrobacterium tumefaciens
PbCl2 at 2 mM, 62% residual activity Agrobacterium tumefaciens
SnCl2 at 2 mM, 62% residual activity Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.55
-
2-coumaranone pH 7.4, 30°C Agrobacterium tumefaciens
2.56
-
3,4-dihydrocoumarin pH 7.4, 30°C Agrobacterium tumefaciens
3.59
-
L-pantoyl lactone pH 7.4, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
gel filtration Agrobacterium tumefaciens
26500
-
2 * 26500, SDS-PAGE Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens AKU 316
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-coumaranone + H2O 25% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens ?
-
?
2-coumaranone + H2O 25% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens AKU 316 ?
-
?
L-pantoyl lactone + H2O stereospecific hydrolysis Agrobacterium tumefaciens L-pantoic acid
-
?
L-pantoyl lactone + H2O stereospecific hydrolysis Agrobacterium tumefaciens AKU 316 L-pantoic acid
-
?
3,4-dihydrocoumarin + H2O 23% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens ?
-
?
3,4-dihydrocoumarin + H2O 23% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens AKU 316 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26500, SDS-PAGE Agrobacterium tumefaciens
More amino acid sequence analysis Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable below Agrobacterium tumefaciens
70
-
20 min, 54% residual activity Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
4
-
no activity below Agrobacterium tumefaciens