Literature summary for 3.1.1.24 extracted from

Okamura-Abe, Y.; Abe, T.; Nishimura, K.; Kawata, Y.; Sato-Izawa, K.; Otsuka, Y.; Nakamura, M.; Kajita, S.; Masai, E.; Sonoki, T.; Katayama, Y.
Beta-ketoadipic acid and muconolactone production from a lignin-related aromatic compound through the protocatechuate 3,4-metabolic pathway (2016), J. Biosci. Bioeng., 121, 652-658 .

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Protein Variants

Protein Variants	Comment	Organism
additional information	preparation of beta-ketoadipate:succinyl-coenzyme A transferase (PcaJ)- and beta-ketoadipate enol lactone hydrolase (PcaD)-inactivated mutants. A double mutant strain shows no beta-ketoadipate:succinyl-coenzyme A transferase and beta-ketoadipate enol lactone hydrolase activties and is unable to assimilate vanillate (VA) and protocatechuate (PCA), and is designated as Pseudomonas putida strain KT2440-dJ, phenotype, overview	Pseudomonas putida

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	Q88N36	-	-
Pseudomonas putida ATCC 47054	Q88N36	-	-
Pseudomonas putida DSM 6125	Q88N36	-	-
Pseudomonas putida NCIMB 11950	Q88N36	-	-

Synonyms

Synonyms	Comment	Organism
beta-ketoadipate enol-lactone hydrolase	-	Pseudomonas putida
PcaD	-	Pseudomonas putida

General Information

General Information	Comment	Organism
malfunction	PcaJ (beta-ketoadipate:succinyl-coenzyme A transferase) inactivation results in production of 3-oxoadipate from protocatechuic acid. The mutant is a biocatalyst to produce both 3-oxoadipate and muconolactone from a lignin-related aromatic compound, a portion of the produced 3-oxoadipate is converted to levulinic acid through a purification step consisting of extraction from the culture and recrystallization. Muconolactone is purified from the culture of the PcaD-inactivated mutant of KT2440, although the isomeric beta-ketoadipate enol-lactone is supposed to be produced because it is the substrate of PcaD	Pseudomonas putida
metabolism	the enzyme is part of the metabolic pathway for 4-hydroxphenyl and guaiacyl lignin-related aromatic compounds in Pseudomonas putida strain KT2440. 3-Carboxymuconolactone (CML), muconolactone (ML), and betaketoadipate enol-latone (KEL), i.e. 3-oxoadipate, are intermediates observed in the metabolism of lignin-related aromatics	Pseudomonas putida

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Release 2023.1 (January 2023)