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Literature summary for 3.1.1.22 extracted from

  • Shirakura, Y.; Fukui, T.; Tanio, T.; Nakayama, K.; Matsuno, R.; Tomita, K.
    An extracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Alcaligenes faecalis (1983), Biochim. Biophys. Acta, 748, 331-339.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate
-
Alcaligenes faecalis
1.5
-
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate
-
Alcaligenes faecalis
4
-
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate
-
Alcaligenes faecalis
11.1
-
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate
-
Alcaligenes faecalis
5.2
-
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
Alcaligenes faecalis
32.8
-
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
Alcaligenes faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Alcaligenes faecalis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000 74000 gel filtration, SDS-PAGE Alcaligenes faecalis
7200
-
1 * 7200, SDS-PAGE Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate Alcaligenes faecalis degradation of poly-(3-hydroxybutyrate) ?
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Alcaligenes faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.2
-
-
Alcaligenes faecalis

Storage Stability

Storage Stability Organism
-20°C, pH 7.5, 1 year Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((S)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[L-(+)-hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
?
(S)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. L-(+)-3-[D-(-)-hydroxybutuyryloxy]butyric acid Alcaligenes faecalis (R)-3-hydroxybutanoate + (S)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O exo-type hydrolysis from the carboxyl terminus Alcaligenes faecalis (R)-3-hydroxybutanoate
-
?
(R)-3-((R)-3-hydroxybutanoyloxy)3butanoate degradation of poly-(3-hydroxybutyrate) Alcaligenes faecalis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 7200, SDS-PAGE Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Alcaligenes faecalis