Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | 98% inhibition at 1 mM | Paracoccus denitrificans | |
iodoacetamide | 11% inhibition at 1 mM | Paracoccus denitrificans | |
NEM | 13% inhibition at 1 mM | Paracoccus denitrificans | |
PMSF | 92% inhibition at 1 mM | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate | pH 8.0, 30°C | Paracoccus denitrificans | |
1 | - |
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate | pH 8.0, 30°C | Paracoccus denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Paracoccus denitrificans |
31000 | - |
1 * 31000, SDS-PAGE | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2350fold, to homogeneity | Paracoccus denitrificans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:ethanol-grown cell | up to the mid-exponential growth phase, used for purification purpose | Paracoccus denitrificans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Paracoccus denitrificans |
14.1 | - |
purified enzyme | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O | - |
Paracoccus denitrificans | (R)-3-hydroxybutanoate | - |
? | |
(R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O | preferred substrate | Paracoccus denitrificans | (R)-3-hydroxybutanoate | - |
? | |
4-nitrophenyl acetate + H2O | 75% of the activity with 4-nitrophenyl butyrate | Paracoccus denitrificans | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | best 4-nitrophenyl ester substrate | Paracoccus denitrificans | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | 43% of the activity with 4-nitrophenyl butyrate | Paracoccus denitrificans | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | 60% of the activity with 4-nitrophenyl butyrate | Paracoccus denitrificans | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propionate + H2O | 81% of the activity with 4-nitrophenyl butyrate | Paracoccus denitrificans | 4-nitrophenol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 31000, SDS-PAGE | Paracoccus denitrificans |
Synonyms | Comment | Organism |
---|---|---|
3HB oligomer hydrolase | - |
Paracoccus denitrificans |
D-(-)-3-hydroxybutyrate oligomer hydrolase | - |
Paracoccus denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paracoccus denitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | 15 min, 80% activity | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paracoccus denitrificans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paracoccus denitrificans | isoelectric focusing | - |
5.2 |