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Literature summary for 3.1.1.20 extracted from

  • Mohapatra, P.K.; Mondal, K.C.; Pati, B.R.
    Production of tannase by the immobilized cells of Bacillus licheniformis KBR6 in Ca-alginate beads (2007), J. Appl. Microbiol., 102, 1462-1467.
    View publication on PubMed
Search for more literature for 3.1.1.20

Application

Application Comment Organism
food industry the enzyme is found to be useful in the manufacture of instant tea, acron wine, coffee-flavoured soft drinks, clarification of beer and fruit juices Bacillus licheniformis

Protein Variants

Protein Variants Comment Organism
additional information active cells of strain KBR6 are immobilized in calcium-alginate and used for the production of tannase, the enzyme production rate is 1.7fold higher than that obtained by free cells, the highest activity is achieved at the third repeated cycle, ten cycles are possible without significant loss of activity Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Bacillus licheniformis
-
 gallate + D-glucose
-
 ?
tannic acid + H2O Bacillus licheniformis KBR6
-
 gallate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
 inducible enzyme
-
Bacillus licheniformis KBR6
-
 inducible enzyme
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyses the hydrolysis of ester and depside bonds in hydrolysable tannin such as tannic acid, releasing glucose and gallic acid Bacillus licheniformis ?
-
 ?
additional information the enzyme catalyses the hydrolysis of ester and depside bonds in hydrolysable tannin such as tannic acid, releasing glucose and gallic acid Bacillus licheniformis KBR6 ?
-
 ?
tannic acid + H2O
-
 Bacillus licheniformis gallate + D-glucose
-
 ?
tannic acid + H2O
-
 Bacillus licheniformis KBR6 gallate + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin-acyl-hydrolase
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Bacillus licheniformis