Application | Comment | Organism |
---|---|---|
biotechnology | immobilization of the enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core improves the thermal and pH stability significantly | Aspergillus niger |
biotechnology | immobilization of enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core. Yield is 36.8% of initial enzyme activity, pH stability and thermal stability improve after microencapsulation. Enzyme can be used for up to 15 runs | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Aspergillus niger | |
0.000011 | - |
Tannic acid | free enzyme, pH 5.0, 30°C | Aspergillus niger | |
0.000041 | - |
Tannic acid | immobilized enzyme, pH 6.0, 40°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
310000 | - |
- |
Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus niger | the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters | ? | - |
? | |
tannic acid + H2O | Aspergillus niger | - |
10 gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
380 | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
digallate + H2O | - |
Aspergillus niger | gallate | - |
? | |
additional information | the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters | Aspergillus niger | ? | - |
? | |
tannic acid + H2O | - |
Aspergillus niger | 10 gallate + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
immobilized enzyme | Aspergillus niger |
30 | - |
free enzyme | Aspergillus niger |
40 | - |
immobilized enzyme | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | immobilized enzyme, 87% remaining activity after 1 h at 60°C | Aspergillus niger |
30 | 45 | immobilized enzyme, 30% remaining activity after 1 h at 60°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
free enzyme | Aspergillus niger |
6 | - |
immobilized enzyme | Aspergillus niger |