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Literature summary for 3.1.1.20 extracted from

  • Yu, X.W.; Li, Y.Q.; Wang, C.X.; Wu, D.
    Immobilization of tannase by microencapsulation and its kinetic characteristics (2003), Biotechnol. Appl. Biochem., 40, 151-155.
    View publication on PubMed

Application

Application Comment Organism
biotechnology immobilization of the enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core improves the thermal and pH stability significantly Aspergillus niger
biotechnology immobilization of enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core. Yield is 36.8% of initial enzyme activity, pH stability and thermal stability improve after microencapsulation. Enzyme can be used for up to 15 runs Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Aspergillus niger
0.000011
-
Tannic acid free enzyme, pH 5.0, 30°C Aspergillus niger
0.000041
-
Tannic acid immobilized enzyme, pH 6.0, 40°C Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
310000
-
-
Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus niger the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters ?
-
?
tannic acid + H2O Aspergillus niger
-
10 gallate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
380
-
-
Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
digallate + H2O
-
Aspergillus niger gallate
-
?
additional information the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters Aspergillus niger ?
-
?
tannic acid + H2O
-
Aspergillus niger 10 gallate + D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
immobilized enzyme Aspergillus niger
30
-
free enzyme Aspergillus niger
40
-
immobilized enzyme Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 immobilized enzyme, 87% remaining activity after 1 h at 60°C Aspergillus niger
30 45 immobilized enzyme, 30% remaining activity after 1 h at 60°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
free enzyme Aspergillus niger
6
-
immobilized enzyme Aspergillus niger