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Literature summary for 3.1.1.2 extracted from
- Temperature dependence of binding and catalysis for human serum arylesterase/paraoxonase (2014), Biochimie, 97, 72-77.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | all kinetic parameters increase with temperature, but the ratios kcat/Km and Kp/Km remain practically constant | Homo sapiens | |
| 0.68 | - |
phenyl acetate | pH 8.0, 10°C | Homo sapiens |  |
| 0.97 | - |
phenyl acetate | pH 8.0, 15°C | Homo sapiens | |
| 1.05 | - |
phenyl acetate | pH 8.0, 20°C | Homo sapiens | |
| 1.33 | - |
phenyl acetate | pH 8.0, 25°C | Homo sapiens | |
| 1.8 | - |
phenyl acetate | pH 8.0, 30°C | Homo sapiens | |
| 2.07 | - |
phenyl acetate | pH 8.0, 35°C | Homo sapiens | |
| 3.03 | - |
phenyl acetate | pH 8.0, 40°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the rate-limiting step is the nucleophilic attack of the carbonyl group of the substrate by a water molecule. The active site is preorganized with no induced fit, and the enzyme-bound calcium plays an important role in stabilizing both the substrate and the transition state | Homo sapiens | ? | - |
? | |
| phenyl acetate + H2O | - |
Homo sapiens | phenol + acetate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | all kinetic parameters increase with temperature, but the ratios kcat/Km and Kp/Km remain practically constant | Homo sapiens | |
| 454 | - |
phenyl acetate | pH 8.0, 10°C | Homo sapiens | |
| 503 | - |
phenyl acetate | pH 8.0, 15°C | Homo sapiens | |
| 659 | - |
phenyl acetate | pH 8.0, 20°C | Homo sapiens | |
| 796 | - |
phenyl acetate | pH 8.0, 25°C | Homo sapiens | |
| 1200 | - |
phenyl acetate | pH 8.0, 30°C | Homo sapiens | |
| 1541 | - |
phenyl acetate | pH 8.0, 35°C | Homo sapiens | |
| 1901 | - |
phenyl acetate | pH 8.0, 40°C | Homo sapiens | |
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