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Literature summary for 3.1.1.15 extracted from

  • Sutter, J.; Johnsen, U.; Schönheit, P.
    Characterization of a pentonolactonase involved in D-xylose and L-arabinose catabolism in the haloarchaeon Haloferax volcanii (2017), FEMS Microbiol. Lett., 364, fnx140 .
    View publication on PubMed
Search for more literature for 3.1.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.9
-
 D-xylono-gamma-lactone pH 7.5, 42°C Haloferax volcanii
37.1
-
 L-arabinono-1,4-lactone pH 7.5, 42°C Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 1 mM, 150% compared to activity with 1 mM MgCl2 Haloferax volcanii
FeCl2 1 mM, 21% compared to activity with 1 mM MgCl2 Haloferax volcanii
KCl the enzyme showed the highest activity at about 1 M KCl and requires divalent cations for activity Haloferax volcanii
MgCl2 Km: 0.2 mM Haloferax volcanii
MnCl2 1 mM, 86% compared to activity with 1 mM MgCl2 Haloferax volcanii
NiCl2 1 mM, 21% compared to activity with 1 mM MgCl2 Haloferax volcanii
ZnCl2 1 mM, 14% compared to activity with 1 mM MgCl2 Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinono-1,4-lactone + H2O Haloferax volcanii
-
 L-arabinonate + H+
-
 ?
L-arabinono-1,4-lactone + H2O Haloferax volcanii DSM 3757
-
 L-arabinonate + H+
-
 ?
L-arabinono-1,4-lactone + H2O Haloferax volcanii ATCC 29605
-
 L-arabinonate + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP31
-
-
Haloferax volcanii ATCC 29605 D4GP31
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactono-gamma-lactone + H2O
-
 Haloferax volcanii ?
-
 ?
D-galactono-gamma-lactone + H2O
-
 Haloferax volcanii DSM 3757 ?
-
 ?
D-galactono-gamma-lactone + H2O
-
 Haloferax volcanii ATCC 29605 ?
-
 ?
D-glucono-delta-lactone + H2O
-
 Haloferax volcanii ?
-
 ?
D-glucono-delta-lactone + H2O
-
 Haloferax volcanii DSM 3757 ?
-
 ?
D-glucono-delta-lactone + H2O
-
 Haloferax volcanii ATCC 29605 ?
-
 ?
D-xylono-gamma-lactone + H2O
-
 Haloferax volcanii xylonate
-
 ?
D-xylono-gamma-lactone + H2O
-
 Haloferax volcanii DSM 3757 xylonate
-
 ?
D-xylono-gamma-lactone + H2O
-
 Haloferax volcanii ATCC 29605 xylonate
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii L-arabinonate
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii DSM 3757 L-arabinonate
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii ATCC 29605 L-arabinonate
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii L-arabinonate + H+
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii DSM 3757 L-arabinonate + H+
-
 ?
L-arabinono-1,4-lactone + H2O
-
 Haloferax volcanii ATCC 29605 L-arabinonate + H+
-
 ?

Synonyms

Synonyms Comment Organism
XacC
-
 Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Haloferax volcanii

Expression

Organism Comment Expression
Haloferax volcanii the enzyme is transcriptionally upregulated by both D-xylose and L-arabinose mediated by the pentose-specific regulator XacR up

General Information

General Information Comment Organism
metabolism the enzyme is involved in the oxidative pathway of L-arabinose degradation to alpha-ketoglutarate Haloferax volcanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.74
-
 L-arabinono-1,4-lactone pH 7.5, 42°C Haloferax volcanii
20.96
-
 D-xylono-gamma-lactone pH 7.5, 42°C Haloferax volcanii