Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.14 extracted from

  • Shimokawa, K.
    Hydrophobic chromatographic purification of ethylene-enhanced chlorophyllase from Citrus unshiu fruits (1982), Biochemistry, 21, 543-545.
No PubMed abstract available
Search for more literature for 3.1.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00265
-
 chlorophyll a
-
 Citrus unshiu
0.00375
-
 chlorophyll a
-
 Citrus unshiu

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 4 * 27000, SDS-PAGE Citrus unshiu
110000
-
 gel filtration Citrus unshiu

Organism

Organism UniProt Comment Textmining
Citrus unshiu
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrus unshiu

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Citrus unshiu
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Citrus unshiu

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll a + H2O
-
 Citrus unshiu chlorophyllide a + phytol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Citrus unshiu

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Citrus unshiu

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, 50% loss of activity Citrus unshiu

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 with both phosphate buffer and borate buffer Citrus unshiu

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.1 50% of maximal activity at pH 6.2 and 8.1 Citrus unshiu