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Literature summary for 3.1.1.14 extracted from

  • Sytykiewicz, H.; Sprawka, I.; Czerniewicz, P.; Sempruch, C.; Leszczy?ski, B.; Sikora, M.
    Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study (2013), Acta Biochim. Pol., 60, 457-465.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus domestica
2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus padus
2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus cerasus
dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus domestica
dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus padus
dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus cerasus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 82% residual activity at 10 mM Prunus cerasus
Ag+ 89% residual activity at 10 mM Prunus domestica
Ag+ 85% residual activity at 10 mM Prunus padus
Al3+ 52% residual activity at 10 mM Prunus cerasus
Al3+ 56% residual activity at 10 mM Prunus domestica
Al3+ 49% residual activity at 10 mM Prunus padus
Bi3+ 52% residual activity at 10 mM Prunus cerasus
Bi3+ 58% residual activity at 10 mM Prunus domestica
Bi3+ 55% residual activity at 10 mM Prunus padus
Co2+ 75% residual activity at 10 mM Prunus cerasus
Co2+ 78% residual activity at 10 mM Prunus domestica
Co2+ 72% residual activity at 10 mM Prunus padus
Cu2+ 39% residual activity at 10 mM Prunus cerasus
Cu2+ 42% residual activity at 10 mM Prunus domestica
Cu2+ 34% residual activity at 10 mM Prunus padus
diethyldicarbonate 83% residual activity at 10 mM Prunus cerasus
diethyldicarbonate 70% residual activity at 10 mM Prunus domestica
diethyldicarbonate 72% residual activity at 10 mM Prunus padus
Fe2+ 31% residual activity at 10 mM Prunus cerasus
Fe2+ 29% residual activity at 10 mM Prunus domestica
Fe2+ 35% residual activity at 10 mM Prunus padus
Fe3+ 73% residual activity at 10 mM Prunus cerasus
Fe3+ 71% residual activity at 10 mM Prunus domestica
Fe3+ 78% residual activity at 10 mM Prunus padus
Hg2+ 22% residual activity at 10 mM Prunus cerasus
Hg2+ 25% residual activity at 10 mM Prunus domestica
Hg2+ 20% residual activity at 10 mM Prunus padus
Mn2+ 72% residual activity at 10 mM Prunus cerasus
Mn2+ 78% residual activity at 10 mM Prunus domestica
Mn2+ 73% residual activity at 10 mM Prunus padus
phenylmethylsulfonyl fluoride 69% residual activity at 10 mM Prunus cerasus
phenylmethylsulfonyl fluoride 58% residual activity at 10 mM Prunus domestica
phenylmethylsulfonyl fluoride 65% residual activity at 10 mM Prunus padus
Zn2+ 51% residual activity at 10 mM Prunus cerasus
Zn2+ 59% residual activity at 10 mM Prunus domestica
Zn2+ 54% residual activity at 10 mM Prunus padus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
0.003
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
0.0031
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
0.0034
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
0.0035
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica
0.0035
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
0.0037
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
0.0038
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
0.0039
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica
0.0042
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
0.0048
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica
0.0061
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus domestica
Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus padus
Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus cerasus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, isoform Chlase F2, SDS-PAGE Prunus domestica
40000
-
x * 40000, isoform Chlase F2, SDS-PAGE Prunus padus
60000
-
x * 60000, isoform Chlase F2, SDS-PAGE Prunus cerasus
90000
-
x * 90000, isoform Chlase F1, SDS-PAGE Prunus padus
100000
-
x * 100000, isoform Chlase F1, SDS-PAGE Prunus domestica
100000
-
x * 100000, isoform Chlase F1, SDS-PAGE Prunus cerasus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyll a + H2O Prunus domestica
-
phytol + chlorophyllide a
-
?
chlorophyll a + H2O Prunus padus
-
phytol + chlorophyllide a
-
?
chlorophyll a + H2O Prunus cerasus
-
phytol + chlorophyllide a
-
?
chlorophyll b + H2O Prunus domestica
-
phytol + chlorophyllide b
-
?
chlorophyll b + H2O Prunus padus
-
phytol + chlorophyllide b
-
?
chlorophyll b + H2O Prunus cerasus
-
phytol + chlorophyllide b
-
?

Organism

Organism UniProt Comment Textmining
Prunus cerasus
-
sour cherry
-
Prunus domestica
-
European plum
-
Prunus padus
-
bird cherry
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus domestica
ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus padus
ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus cerasus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Prunus domestica
-
leaf
-
Prunus padus
-
leaf
-
Prunus cerasus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
unpurified homogenate, at pH 7.0 and 30°C Prunus padus
0.51
-
unpurified homogenate, at pH 7.0 and 30°C Prunus cerasus
0.68
-
unpurified homogenate, at pH 7.0 and 30°C Prunus domestica
5.53
-
after 3.73fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
7.42
-
after 3.73fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
9.52
-
after 18.67fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
14.75
-
after 28.92fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
16.92
-
after 24.88fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica
19.47
-
after 28.63fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyll a + H2O
-
Prunus domestica phytol + chlorophyllide a
-
?
chlorophyll a + H2O
-
Prunus padus phytol + chlorophyllide a
-
?
chlorophyll a + H2O
-
Prunus cerasus phytol + chlorophyllide a
-
?
chlorophyll b + H2O
-
Prunus domestica phytol + chlorophyllide b
-
?
chlorophyll b + H2O
-
Prunus padus phytol + chlorophyllide b
-
?
chlorophyll b + H2O
-
Prunus cerasus phytol + chlorophyllide b
-
?

Subunits

Subunits Comment Organism
? x * 100000, isoform Chlase F1, SDS-PAGE Prunus domestica
? x * 100000, isoform Chlase F1, SDS-PAGE Prunus cerasus
? x * 35000, isoform Chlase F2, SDS-PAGE Prunus domestica
? x * 40000, isoform Chlase F2, SDS-PAGE Prunus padus
? x * 60000, isoform Chlase F2, SDS-PAGE Prunus cerasus
? x * 90000, isoform Chlase F1, SDS-PAGE Prunus padus

Synonyms

Synonyms Comment Organism
CHlase F1 isoform Prunus domestica
CHlase F1 isoform Prunus padus
CHlase F1 isoform Prunus cerasus
Chlase F2 isoform Prunus domestica
Chlase F2 isoform Prunus padus
Chlase F2 isoform Prunus cerasus
chlorophyll chlorophyllido-hydrolase
-
Prunus domestica
chlorophyll chlorophyllido-hydrolase
-
Prunus padus
chlorophyll chlorophyllido-hydrolase
-
Prunus cerasus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Prunus domestica
7.8
-
-
Prunus padus
8
-
-
Prunus cerasus