Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | subtle stimulation of enzymatic activity at 10 mM | Prunus domestica | |
2-mercaptoethanol | subtle stimulation of enzymatic activity at 10 mM | Prunus padus | |
2-mercaptoethanol | subtle stimulation of enzymatic activity at 10 mM | Prunus cerasus | |
dithiothreitol | subtle stimulation of enzymatic activity at 10 mM | Prunus domestica | |
dithiothreitol | subtle stimulation of enzymatic activity at 10 mM | Prunus padus | |
dithiothreitol | subtle stimulation of enzymatic activity at 10 mM | Prunus cerasus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 82% residual activity at 10 mM | Prunus cerasus | |
Ag+ | 89% residual activity at 10 mM | Prunus domestica | |
Ag+ | 85% residual activity at 10 mM | Prunus padus | |
Al3+ | 52% residual activity at 10 mM | Prunus cerasus | |
Al3+ | 56% residual activity at 10 mM | Prunus domestica | |
Al3+ | 49% residual activity at 10 mM | Prunus padus | |
Bi3+ | 52% residual activity at 10 mM | Prunus cerasus | |
Bi3+ | 58% residual activity at 10 mM | Prunus domestica | |
Bi3+ | 55% residual activity at 10 mM | Prunus padus | |
Co2+ | 75% residual activity at 10 mM | Prunus cerasus | |
Co2+ | 78% residual activity at 10 mM | Prunus domestica | |
Co2+ | 72% residual activity at 10 mM | Prunus padus | |
Cu2+ | 39% residual activity at 10 mM | Prunus cerasus | |
Cu2+ | 42% residual activity at 10 mM | Prunus domestica | |
Cu2+ | 34% residual activity at 10 mM | Prunus padus | |
diethyldicarbonate | 83% residual activity at 10 mM | Prunus cerasus | |
diethyldicarbonate | 70% residual activity at 10 mM | Prunus domestica | |
diethyldicarbonate | 72% residual activity at 10 mM | Prunus padus | |
Fe2+ | 31% residual activity at 10 mM | Prunus cerasus | |
Fe2+ | 29% residual activity at 10 mM | Prunus domestica | |
Fe2+ | 35% residual activity at 10 mM | Prunus padus | |
Fe3+ | 73% residual activity at 10 mM | Prunus cerasus | |
Fe3+ | 71% residual activity at 10 mM | Prunus domestica | |
Fe3+ | 78% residual activity at 10 mM | Prunus padus | |
Hg2+ | 22% residual activity at 10 mM | Prunus cerasus | |
Hg2+ | 25% residual activity at 10 mM | Prunus domestica | |
Hg2+ | 20% residual activity at 10 mM | Prunus padus | |
Mn2+ | 72% residual activity at 10 mM | Prunus cerasus | |
Mn2+ | 78% residual activity at 10 mM | Prunus domestica | |
Mn2+ | 73% residual activity at 10 mM | Prunus padus | |
phenylmethylsulfonyl fluoride | 69% residual activity at 10 mM | Prunus cerasus | |
phenylmethylsulfonyl fluoride | 58% residual activity at 10 mM | Prunus domestica | |
phenylmethylsulfonyl fluoride | 65% residual activity at 10 mM | Prunus padus | |
Zn2+ | 51% residual activity at 10 mM | Prunus cerasus | |
Zn2+ | 59% residual activity at 10 mM | Prunus domestica | |
Zn2+ | 54% residual activity at 10 mM | Prunus padus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
chlorophyll a | isoform Chlase F1, at pH 7.0 and 30°C | Prunus padus | |
0.003 | - |
chlorophyll b | isoform Chlase F1, at pH 7.0 and 30°C | Prunus padus | |
0.0031 | - |
chlorophyll a | isoform Chlase F2, at pH 7.0 and 30°C | Prunus padus | |
0.0034 | - |
chlorophyll a | isoform Chlase F1, at pH 7.0 and 30°C | Prunus cerasus | |
0.0035 | - |
chlorophyll a | isoform Chlase F1, at pH 7.0 and 30°C | Prunus domestica | |
0.0035 | - |
chlorophyll b | isoform Chlase F2, at pH 7.0 and 30°C | Prunus padus | |
0.0037 | - |
chlorophyll b | isoform Chlase F1, at pH 7.0 and 30°C | Prunus cerasus | |
0.0038 | - |
chlorophyll b | isoform Chlase F2, at pH 7.0 and 30°C | Prunus cerasus | |
0.0039 | - |
chlorophyll b | isoform Chlase F1, at pH 7.0 and 30°C | Prunus domestica | |
0.0042 | - |
chlorophyll a | isoform Chlase F2, at pH 7.0 and 30°C | Prunus cerasus | |
0.0048 | - |
chlorophyll a | isoform Chlase F2, at pH 7.0 and 30°C | Prunus domestica | |
0.0061 | - |
chlorophyll b | isoform Chlase F2, at pH 7.0 and 30°C | Prunus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | subtle stimulation of enzymatic activity at 10 mM | Prunus domestica | |
Mg2+ | subtle stimulation of enzymatic activity at 10 mM | Prunus padus | |
Mg2+ | subtle stimulation of enzymatic activity at 10 mM | Prunus cerasus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, isoform Chlase F2, SDS-PAGE | Prunus domestica |
40000 | - |
x * 40000, isoform Chlase F2, SDS-PAGE | Prunus padus |
60000 | - |
x * 60000, isoform Chlase F2, SDS-PAGE | Prunus cerasus |
90000 | - |
x * 90000, isoform Chlase F1, SDS-PAGE | Prunus padus |
100000 | - |
x * 100000, isoform Chlase F1, SDS-PAGE | Prunus domestica |
100000 | - |
x * 100000, isoform Chlase F1, SDS-PAGE | Prunus cerasus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll a + H2O | Prunus domestica | - |
phytol + chlorophyllide a | - |
? | |
chlorophyll a + H2O | Prunus padus | - |
phytol + chlorophyllide a | - |
? | |
chlorophyll a + H2O | Prunus cerasus | - |
phytol + chlorophyllide a | - |
? | |
chlorophyll b + H2O | Prunus domestica | - |
phytol + chlorophyllide b | - |
? | |
chlorophyll b + H2O | Prunus padus | - |
phytol + chlorophyllide b | - |
? | |
chlorophyll b + H2O | Prunus cerasus | - |
phytol + chlorophyllide b | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus cerasus | - |
sour cherry | - |
Prunus domestica | - |
European plum | - |
Prunus padus | - |
bird cherry | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sephadex G-200gel filtration | Prunus domestica |
ammonium sulfate precipitation and Sephadex G-200gel filtration | Prunus padus |
ammonium sulfate precipitation and Sephadex G-200gel filtration | Prunus cerasus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Prunus domestica | - |
leaf | - |
Prunus padus | - |
leaf | - |
Prunus cerasus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
unpurified homogenate, at pH 7.0 and 30°C | Prunus padus |
0.51 | - |
unpurified homogenate, at pH 7.0 and 30°C | Prunus cerasus |
0.68 | - |
unpurified homogenate, at pH 7.0 and 30°C | Prunus domestica |
5.53 | - |
after 3.73fold purification, isoform Chlase F1, at pH 7.0 and 30°C | Prunus padus |
7.42 | - |
after 3.73fold purification, isoform Chlase F2, at pH 7.0 and 30°C | Prunus padus |
9.52 | - |
after 18.67fold purification, isoform Chlase F2, at pH 7.0 and 30°C | Prunus cerasus |
14.75 | - |
after 28.92fold purification, isoform Chlase F1, at pH 7.0 and 30°C | Prunus cerasus |
16.92 | - |
after 24.88fold purification, isoform Chlase F2, at pH 7.0 and 30°C | Prunus domestica |
19.47 | - |
after 28.63fold purification, isoform Chlase F1, at pH 7.0 and 30°C | Prunus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll a + H2O | - |
Prunus domestica | phytol + chlorophyllide a | - |
? | |
chlorophyll a + H2O | - |
Prunus padus | phytol + chlorophyllide a | - |
? | |
chlorophyll a + H2O | - |
Prunus cerasus | phytol + chlorophyllide a | - |
? | |
chlorophyll b + H2O | - |
Prunus domestica | phytol + chlorophyllide b | - |
? | |
chlorophyll b + H2O | - |
Prunus padus | phytol + chlorophyllide b | - |
? | |
chlorophyll b + H2O | - |
Prunus cerasus | phytol + chlorophyllide b | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 100000, isoform Chlase F1, SDS-PAGE | Prunus domestica |
? | x * 100000, isoform Chlase F1, SDS-PAGE | Prunus cerasus |
? | x * 35000, isoform Chlase F2, SDS-PAGE | Prunus domestica |
? | x * 40000, isoform Chlase F2, SDS-PAGE | Prunus padus |
? | x * 60000, isoform Chlase F2, SDS-PAGE | Prunus cerasus |
? | x * 90000, isoform Chlase F1, SDS-PAGE | Prunus padus |
Synonyms | Comment | Organism |
---|---|---|
CHlase F1 | isoform | Prunus domestica |
CHlase F1 | isoform | Prunus padus |
CHlase F1 | isoform | Prunus cerasus |
Chlase F2 | isoform | Prunus domestica |
Chlase F2 | isoform | Prunus padus |
Chlase F2 | isoform | Prunus cerasus |
chlorophyll chlorophyllido-hydrolase | - |
Prunus domestica |
chlorophyll chlorophyllido-hydrolase | - |
Prunus padus |
chlorophyll chlorophyllido-hydrolase | - |
Prunus cerasus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Prunus domestica |
7.8 | - |
- |
Prunus padus |
8 | - |
- |
Prunus cerasus |