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Literature summary for 3.1.1.13 extracted from

  • Brown, A.; Hang, J.; Dussault, P.; Carr, T.
    Plant sterol and stanol substrate specificity of pancreatic cholesterol esterase (2010), J. Nutr. Biochem., 21, 736-740.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
4°C, 100 mM potassium phosphate buffer at pH 7.0, up to 7 days, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl oleate + H2O 100% activity, best substrate Sus scrofa cholesterol + oleate
-
?
cholesteryl palmitate + H2O about 50% activity compared to cholesteryl oleate Sus scrofa cholesterol + palmitate
-
?
cholesteryl stearate + H2O about 45% activity compared to cholesteryl oleate Sus scrofa cholesterol + stearate
-
?
sitosterol oleate + H2O about 60% activity compared to cholesteryl oleate Sus scrofa sitosterol + oleate
-
?
sitosterol palmitate + H2O about 30% activity compared to cholesteryl oleate Sus scrofa sitosterol + palmitate
-
?
sitosterol stearate + H2O about 30% activity compared to cholesteryl oleate Sus scrofa sitosterol + stearate
-
?
stigmastanol oleate + H2O about 65% activity compared to cholesteryl oleate Sus scrofa stigmastanol + oleate
-
?
stigmastanol palmitate + H2O about 30% activity compared to cholesteryl oleate Sus scrofa stigmastanol + palmitate
-
?
stigmastanol stearate + H2O about 20% activity compared to cholesteryl oleate Sus scrofa stigmastanol + stearate
-
?
stigmasterol oleate + H2O about 35% activity compared to cholesteryl oleate Sus scrofa stigmasterol + oleate
-
?
stigmasterol palmitate + H2O about 15% activity compared to cholesteryl oleate Sus scrofa stigmasterol + palmitate
-
?
stigmasterol stearate + H2O about 15% activity compared to cholesteryl oleate Sus scrofa stigmasterol + stearate
-
?

Synonyms

Synonyms Comment Organism
pancreatic cholesterol esterase
-
Sus scrofa
PCE
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 8 cholesteryl oleate is most effectively hydrolyzed at pH 7.0, with pH 6.0, 6.5, 7.5 and 8.0 retaining 89%, 80%, 84% and 72% activity, respectively Sus scrofa