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Literature summary for 3.1.1.13 extracted from

  • Du, L.; Huo, Y.; Ge, F.; Yu, J.; Li, W.; Cheng, G.; Yong, B.; Zeng, L.; Huang, M.
    Purification and characterization of novel extracellular cholesterol esterase from Acinetobacter sp. (2010), J. Basic Microbiol., 50 Suppl 1, S30-S36.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
boracic acid 106.4% activity at 1 mM Acinetobacter sp.
additional information enzymatic activity is not significantly affected by EDTA, sodium cholate, Triton X100 and beta-mercaptoethanol Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 23.4% residual activity at 1 mM Acinetobacter sp.
dithiothreitol 65.3% residual activity at 1 mM Acinetobacter sp.
Hg2+ 22.9% residual activity at 1 mM Acinetobacter sp.
additional information not inhibited by phenylmethylsulfonyl fluoride, (1 mM) Acinetobacter sp.
Na+ 93% residual activity at mM Acinetobacter sp.
NaN3 93.6% residual activity at 1 mM Acinetobacter sp.
SDS 50.3% residual activity at 0.1% (w/v) Acinetobacter sp.
Tween 80 90.1% residual activity at 1 mM Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.536
-
cholesteryl linoleate at pH 7.0, 37°C Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 105.2% activity at 1 mM Acinetobacter sp.
additional information not influenced by Co2+, Cu2+, Mg2+, and Li+ Acinetobacter sp.
Zn2+ 105.8% activity at 1 mM Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6500
-
gel filtration Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. CHE4-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration (19.4% protein recovery with 11.5fold purification) Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
culture supernatant, at pH 7.0, 37°C Acinetobacter sp.
11.9
-
after 18fold purification, at pH 7.0, 37°C Acinetobacter sp.

Storage Stability

Storage Stability Organism
25°C, in potassium phosphate buffer (pH 7.0), 30 days, 14.8% loss of activity Acinetobacter sp.
4°C, in potassium phosphate buffer (pH 7.0), 30 days, 3.5% loss of activity Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O
-
Acinetobacter sp. cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Acinetobacter sp. CHE4-1 cholesterol + acetate
-
?
cholesteryl butyrate + H2O
-
Acinetobacter sp. cholesterol + butyrate
-
?
cholesteryl caproate + H2O
-
Acinetobacter sp. cholesterol + caproate
-
?
cholesteryl laurate + H2O
-
Acinetobacter sp. cholesterol + laurate
-
?
cholesteryl linoleate + H2O best substrate Acinetobacter sp. cholesterol + linoleate
-
?
cholesteryl linoleate + H2O best substrate Acinetobacter sp. CHE4-1 cholesterol + linoleate
-
?
cholesteryl oleate + H2O
-
Acinetobacter sp. cholesterol + oleate
-
?
cholesteryl oleate + H2O
-
Acinetobacter sp. CHE4-1 cholesterol + oleate
-
?
cholesteryl palmitate + H2O
-
Acinetobacter sp. cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Acinetobacter sp. CHE4-1 cholesterol + palmitate
-
?
additional information rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate Acinetobacter sp. ?
-
?
additional information rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate Acinetobacter sp. CHE4-1 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 6500, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
ChE
-
Acinetobacter sp.
cholesterol esterase
-
Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Acinetobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60
-
Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 the enzyme loses about 81.8% of its activity after incubation at 60°C for 30 min, and loses all its activity after incubation at 70°C for 10 min Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Acinetobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the enzyme is stable (100% activity) after 1 h at pH 5.5-8.5, the enzyme maintains about 80% activity after 1 h at pH 4.0-5.5 and at pH 8.5-10.0 Acinetobacter sp.