Activating Compound | Comment | Organism | Structure |
---|---|---|---|
boracic acid | 106.4% activity at 1 mM | Acinetobacter sp. | |
additional information | enzymatic activity is not significantly affected by EDTA, sodium cholate, Triton X100 and beta-mercaptoethanol | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 23.4% residual activity at 1 mM | Acinetobacter sp. | |
dithiothreitol | 65.3% residual activity at 1 mM | Acinetobacter sp. | |
Hg2+ | 22.9% residual activity at 1 mM | Acinetobacter sp. | |
additional information | not inhibited by phenylmethylsulfonyl fluoride, (1 mM) | Acinetobacter sp. | |
Na+ | 93% residual activity at mM | Acinetobacter sp. | |
NaN3 | 93.6% residual activity at 1 mM | Acinetobacter sp. | |
SDS | 50.3% residual activity at 0.1% (w/v) | Acinetobacter sp. | |
Tween 80 | 90.1% residual activity at 1 mM | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.536 | - |
cholesteryl linoleate | at pH 7.0, 37°C | Acinetobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 105.2% activity at 1 mM | Acinetobacter sp. | |
additional information | not influenced by Co2+, Cu2+, Mg2+, and Li+ | Acinetobacter sp. | |
Zn2+ | 105.8% activity at 1 mM | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
6500 | - |
gel filtration | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. CHE4-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration (19.4% protein recovery with 11.5fold purification) | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
culture supernatant, at pH 7.0, 37°C | Acinetobacter sp. |
11.9 | - |
after 18fold purification, at pH 7.0, 37°C | Acinetobacter sp. |
Storage Stability | Organism |
---|---|
25°C, in potassium phosphate buffer (pH 7.0), 30 days, 14.8% loss of activity | Acinetobacter sp. |
4°C, in potassium phosphate buffer (pH 7.0), 30 days, 3.5% loss of activity | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl acetate + H2O | - |
Acinetobacter sp. | cholesterol + acetate | - |
? | |
cholesteryl acetate + H2O | - |
Acinetobacter sp. CHE4-1 | cholesterol + acetate | - |
? | |
cholesteryl butyrate + H2O | - |
Acinetobacter sp. | cholesterol + butyrate | - |
? | |
cholesteryl caproate + H2O | - |
Acinetobacter sp. | cholesterol + caproate | - |
? | |
cholesteryl laurate + H2O | - |
Acinetobacter sp. | cholesterol + laurate | - |
? | |
cholesteryl linoleate + H2O | best substrate | Acinetobacter sp. | cholesterol + linoleate | - |
? | |
cholesteryl linoleate + H2O | best substrate | Acinetobacter sp. CHE4-1 | cholesterol + linoleate | - |
? | |
cholesteryl oleate + H2O | - |
Acinetobacter sp. | cholesterol + oleate | - |
? | |
cholesteryl oleate + H2O | - |
Acinetobacter sp. CHE4-1 | cholesterol + oleate | - |
? | |
cholesteryl palmitate + H2O | - |
Acinetobacter sp. | cholesterol + palmitate | - |
? | |
cholesteryl palmitate + H2O | - |
Acinetobacter sp. CHE4-1 | cholesterol + palmitate | - |
? | |
additional information | rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate | Acinetobacter sp. | ? | - |
? | |
additional information | rank of the relative rates of hydrolysis by Acinetobacter sp. strain CHE4-1 enzyme are linoleate > oleate > acetate > palmitate > butyrate > laurate > caproate | Acinetobacter sp. CHE4-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 6500, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
ChE | - |
Acinetobacter sp. |
cholesterol esterase | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Acinetobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | - |
Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the enzyme loses about 81.8% of its activity after incubation at 60°C for 30 min, and loses all its activity after incubation at 70°C for 10 min | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Acinetobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Acinetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzyme is stable (100% activity) after 1 h at pH 5.5-8.5, the enzyme maintains about 80% activity after 1 h at pH 4.0-5.5 and at pH 8.5-10.0 | Acinetobacter sp. |