Activating Compound | Comment | Organism | Structure |
---|---|---|---|
n-hexane | the addition of n-hexane markedly enhances the esterification activities on all the medium- and long-chain saturated fatty acids | Trichoderma sp. |
Organic Solvent | Comment | Organism |
---|---|---|
n-hexane | the enzyme loses 49% of the original activity when exposed to n-hexane for 19 h without agitation | Trichoderma sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma sp. | - |
- |
- |
Trichoderma sp. AS59 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + oleate | 100% esterification with 50 units of enzyme after 20 h. The enzyme is largely independent of water content in the synthesis of cholesteryl oleate, and it achieves near-complete esterification in the presence of an equimolar excess of oleate | Trichoderma sp. | cholesteryl oleate + H2O | - |
? | |
cholesteryl acetate + H2O | - |
Trichoderma sp. | cholesterol + acetate | - |
? | |
cholesteryl acetate + H2O | - |
Trichoderma sp. AS59 | cholesterol + acetate | - |
? | |
cholesteryl butyrate + H2O | - |
Trichoderma sp. | cholesterol + butyrate | - |
? | |
cholesteryl caprate + H2O | - |
Trichoderma sp. | cholesterol + caprate | - |
? | |
cholesteryl caproate + H2O | - |
Trichoderma sp. | cholesterol + caproate | - |
? | |
cholesteryl caprylate + H2O | - |
Trichoderma sp. | cholesterol + caprylate | - |
? | |
cholesteryl decanoate + H2O | - |
Trichoderma sp. | cholesterol + decanoate | - |
? | |
cholesteryl hexanoate + H2O | - |
Trichoderma sp. | cholesterol + hexanoate | - |
? | |
cholesteryl laurate + H2O | - |
Trichoderma sp. | cholesterol + laurate | - |
? | |
cholesteryl myristate + H2O | - |
Trichoderma sp. | cholesterol + myristate | - |
? | |
cholesteryl palmitate + H2O | - |
Trichoderma sp. | cholesterol + palmitate | - |
? | |
cholesteryl palmitate + H2O | - |
Trichoderma sp. AS59 | cholesterol + palmitate | - |
? | |
cholesteryl propionate + H2O | - |
Trichoderma sp. | cholesterol + propionate | - |
? | |
cholesteryl stearate + H2O | - |
Trichoderma sp. | cholesterol + stearate | - |
? | |
cholesteryl stearate + H2O | - |
Trichoderma sp. AS59 | cholesterol + stearate | - |
? | |
additional information | the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification | Trichoderma sp. | ? | - |
? | |
additional information | the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate | Trichoderma sp. | ? | - |
? | |
additional information | the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification | Trichoderma sp. AS59 | ? | - |
? | |
additional information | the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate | Trichoderma sp. AS59 | ? | - |
? | |
stigmasterol + stearate | - |
Trichoderma sp. | stigmasteryl stearate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol esterase | - |
Trichoderma sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | activity decreases gradually with increasing temperature. 90%, 89%, 85%, 85%, and 72% at 20°C, 25°C, 30°C, 35°C, and 40°C in 5 h of incubation at pH 5.0 | Trichoderma sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the degree of esterification declines with increasing pH. 89%, 83%, 76%, and 43% activity at pH 5.0, 6.0, 7.0, and 8.0, respectively, in 5 h of incubation at 20°C | Trichoderma sp. |