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Literature summary for 3.1.1.13 extracted from

  • Morinaga, N.; Maeda, A.; Mizuno, T.; Bunya, M.; Sugihara, S.; Sugihara, A.
    Synthesis of fatty acid sterol esters using cholesterol esterase from Trichoderma sp. AS59 (2011), Enzyme Microb. Technol., 48, 498-504.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
n-hexane the addition of n-hexane markedly enhances the esterification activities on all the medium- and long-chain saturated fatty acids Trichoderma sp.

Organic Solvent Stability

Organic Solvent Comment Organism
n-hexane the enzyme loses 49% of the original activity when exposed to n-hexane for 19 h without agitation Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
-
-
-
Trichoderma sp. AS59
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + oleate 100% esterification with 50 units of enzyme after 20 h. The enzyme is largely independent of water content in the synthesis of cholesteryl oleate, and it achieves near-complete esterification in the presence of an equimolar excess of oleate Trichoderma sp. cholesteryl oleate + H2O
-
?
cholesteryl acetate + H2O
-
Trichoderma sp. cholesterol + acetate
-
?
cholesteryl acetate + H2O
-
Trichoderma sp. AS59 cholesterol + acetate
-
?
cholesteryl butyrate + H2O
-
Trichoderma sp. cholesterol + butyrate
-
?
cholesteryl caprate + H2O
-
Trichoderma sp. cholesterol + caprate
-
?
cholesteryl caproate + H2O
-
Trichoderma sp. cholesterol + caproate
-
?
cholesteryl caprylate + H2O
-
Trichoderma sp. cholesterol + caprylate
-
?
cholesteryl decanoate + H2O
-
Trichoderma sp. cholesterol + decanoate
-
?
cholesteryl hexanoate + H2O
-
Trichoderma sp. cholesterol + hexanoate
-
?
cholesteryl laurate + H2O
-
Trichoderma sp. cholesterol + laurate
-
?
cholesteryl myristate + H2O
-
Trichoderma sp. cholesterol + myristate
-
?
cholesteryl palmitate + H2O
-
Trichoderma sp. cholesterol + palmitate
-
?
cholesteryl palmitate + H2O
-
Trichoderma sp. AS59 cholesterol + palmitate
-
?
cholesteryl propionate + H2O
-
Trichoderma sp. cholesterol + propionate
-
?
cholesteryl stearate + H2O
-
Trichoderma sp. cholesterol + stearate
-
?
cholesteryl stearate + H2O
-
Trichoderma sp. AS59 cholesterol + stearate
-
?
additional information the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification Trichoderma sp. ?
-
?
additional information the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate Trichoderma sp. ?
-
?
additional information the enzyme catalyzes reactions between cholesterol and methyl oleate/triolein. The degree of esterification of cholesterol with methyl oleate is 31% at the most after 19 h. The use of triolein as acyl donor results in lower esterification Trichoderma sp. AS59 ?
-
?
additional information the hydrolytic activity of the enzyme increases from cholesterol acetate to caproate, then decreases sharply from caproate to caprate, with a further gradual decrease through stearate. In esterification there is virtually no activity with short-chain free fatty acids from acetate through caproate, a marked increase with caprylate, and a constant level from caprylate through stearate Trichoderma sp. AS59 ?
-
?
stigmasterol + stearate
-
Trichoderma sp. stigmasteryl stearate + H2O
-
?

Synonyms

Synonyms Comment Organism
cholesterol esterase
-
Trichoderma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 activity decreases gradually with increasing temperature. 90%, 89%, 85%, 85%, and 72% at 20°C, 25°C, 30°C, 35°C, and 40°C in 5 h of incubation at pH 5.0 Trichoderma sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the degree of esterification declines with increasing pH. 89%, 83%, 76%, and 43% activity at pH 5.0, 6.0, 7.0, and 8.0, respectively, in 5 h of incubation at 20°C Trichoderma sp.