Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris, resulting in higher activities than those obtained in the Ophistoma piceae cultures | Ophiostoma piceae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58200 | - |
MALDI-TOF, SDS-PAGE | Ophiostoma piceae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ophiostoma piceae | Q2TFW1 | - |
- |
Purification (Comment) | Organism |
---|---|
The Ophiostoma piceae esterase is purified from 15-day-old cultures on glucose-peptone-yeast extract medium supplemented with 0.5% olive oil. Recombinant protein is purified following the same procedure from 7-day-old cultures growing on BMMY medium | Ophiostoma piceae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79.84 | - |
substrate cholesteryl oleate, native enzyme in the presence of 5% Genapol X-100 | Ophiostoma piceae |
171 | - |
substrate triolein, native enzyme in the presence of 5% Genapol X-100 | Ophiostoma piceae |
319 | - |
substrate cholesteryl oleate, recombinant enzyme in the presence of 5% Genapol X-100 | Ophiostoma piceae |
449.7 | - |
substrate 4-nitrophenyl butyrate, native enzyme in the presence of 1% Genapol X-100 | Ophiostoma piceae |
698.1 | - |
substrate triolein, recombinant enzyme in the presence of 5% Genapol X-100 | Ophiostoma piceae |
1336 | - |
substrate 4-nitrophenyl butyrate, recombinant enzyme in the presence of 1% Genapol X-100 | Ophiostoma piceae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl oleate + H2O | - |
Ophiostoma piceae | cholesterol + oleate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Ophiostoma piceae | p-nitrophenol + butanoic acid | - |
? | |
p-nitrophenyl palmitate + H2O | - |
Ophiostoma piceae | p-nitrophenol + palmitic acid | - |
? | |
tributyrin + H2O | - |
Ophiostoma piceae | dibutyrin + butyrate | - |
? | |
triolein + H2O | - |
Ophiostoma piceae | diolein + oleate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OPE | - |
Ophiostoma piceae |
Sterol esterase | - |
Ophiostoma piceae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Ophiostoma piceae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ophiostoma piceae |