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Literature summary for 3.1.1.13 extracted from

  • Maeda, A.; Mizuno, T.; Bunya, M.; Sugihara, S.; Nakayama, D.; Tsunasawa, S.; Hirota, Y.; Sugihara, A.
    Characterization of novel cholesterol esterase from Trichoderma sp. AS59 with high ability to synthesize steryl esters (2008), J. Biosci. Bioeng., 105, 341-349.
    View publication on PubMed
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
cholate not affected by cholate up to a concentration of 5 mM, but after a gradual decrease of activity at more than 5 mM, the activity re-increases at 40 mM to reach more than twice the basal activity at 100 mM Trichoderma sp.

Inhibitors

Inhibitors Comment Organism Structure
cholate activity decreases gradually from 5 to 40 mM cholate Trichoderma sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Trichoderma sp.
54000
-
 SDS-PAGE Trichoderma sp.
58200
-
 MALDI-TOF mass spectrometry Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
-
-
-
Trichoderma sp. AS59
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Superdex 200 gel filtration, and MacroPrep methyl column chromatography Trichoderma sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.108
-
 crude extract Trichoderma sp.
31.1
-
 after 288fold purification Trichoderma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl acetate + H2O 9% activity compared to linoleate Trichoderma sp. cholesterol + acetate
-
 ?
cholesteryl acetate + H2O 9% activity compared to linoleate Trichoderma sp. AS59 cholesterol + acetate
-
 ?
cholesteryl butyrate + H2O 121% activity compared to linoleate Trichoderma sp. cholesterol + butyrate
-
 ?
cholesteryl caprate + H2O 21% activity compared to linoleate Trichoderma sp. cholesterol + caprate
-
 ?
cholesteryl caproate + H2O 37% activity compared to linoleate Trichoderma sp. cholesterol + caproate
-
 ?
cholesteryl caprylate + H2O 79% activity compared to linoleate Trichoderma sp. cholesterol + caprylate
-
 ?
cholesteryl laurate + H2O 35% activity compared to linoleate Trichoderma sp. cholesterol + laurate
-
 ?
cholesteryl linoleate + H2O 100% activity Trichoderma sp. cholesterol + linoleate
-
 ?
cholesteryl linoleate + H2O 100% activity Trichoderma sp. AS59 cholesterol + linoleate
-
 ?
cholesteryl myristate + H2O 42% activity compared to linoleate Trichoderma sp. cholesterol + myristate
-
 ?
cholesteryl oleate + H2O 21% activity compared to linoleate Trichoderma sp. cholesterol + oleate
-
 ?
cholesteryl oleate + H2O 21% activity compared to linoleate Trichoderma sp. AS59 cholesterol + oleate
-
 ?
cholesteryl palmitate + H2O 38% activity compared to linoleate Trichoderma sp. cholesterol + palmitate
-
 ?
cholesteryl palmitate + H2O 38% activity compared to linoleate Trichoderma sp. AS59 cholesterol + palmitate
-
 ?
cholesteryl stearate + H2O 18% activity compared to linoleate Trichoderma sp. cholesterol + stearate
-
 ?
additional information the enzyme is also active on fatty acid esters of methanol and 4-nitrophenol Trichoderma sp. ?
-
 ?
additional information the enzyme is also active on fatty acid esters of methanol and 4-nitrophenol Trichoderma sp. AS59 ?
-
 ?
stigmasteryl oleate + H2O
-
 Trichoderma sp. stigmasterol + oleate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 58200, MALDI-TOF mass spectrometry Trichoderma sp.

Synonyms

Synonyms Comment Organism
carboxylester lipase
-
 Trichoderma sp.
CEase
-
 Trichoderma sp.
cholesterol esterase
-
 Trichoderma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Trichoderma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 30 the enzyme retains 75% of the initial activity after 18 h at 30°C in the pH range of 3.5-7.5, the enzyme is stable at temperatures below 15°C for at least 18 h but gradually loses activity at higher temperatures Trichoderma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Trichoderma sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5
-
 Trichoderma sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma sp. isoelectric focusing
-
 4.3