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Literature summary for 3.1.1.13 extracted from

  • Takeda, Y.; Aono, R.; Doukyu, N.
    Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200 (2006), Extremophiles, 10, 269-177.
    View publication on PubMed
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
2-propanol 10% v/v, 460% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
acetone 10% v/v, 570% of initial activity, 30% v/v, 53% of initial activity Burkholderia cepacia
DMF 10% v/v, 540% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
DMSO 10% v/v, 500% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
ethanol 10% v/v, 580% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
methanol 10% v/v, 680% of initial activity, 30% v/v, 58% of initial activity Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 72% residual activity Burkholderia cepacia
Cu2+ 1 mM, 61% residual activity Burkholderia cepacia
Hg2+ 1 mM, 11% residual activity Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Burkholderia cepacia

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 10% v/v, 460% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
Acetone 10% v/v, 570% of initial activity, 30% v/v, 53% of initial activity Burkholderia cepacia
dimethyl formamide 10% v/v, 540% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
dimethyl sulfoxide 10% v/v, 500% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
Ethanol 10% v/v, 580% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
Methanol 10% v/v, 680% of initial activity, 30% v/v, 58% of initial activity Burkholderia cepacia
additional information enzyme is highly stable in various water-miscible organic solvents Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia Q75NT4
-
-
Burkholderia cepacia ST-200 Q75NT4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Burkholderia cepacia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1
-
 pH 7.0, 37°C Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol acetate + H2O 3% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + acetic acid
-
 ?
cholesterol acetate + H2O 3% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + acetic acid
-
 ?
cholesterol butyrate + H2O 18% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + butyric acid
-
 ?
cholesterol butyrate + H2O 18% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + butyric acid
-
 ?
cholesterol decanoate + H2O 34% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + decanoic acid
-
 ?
cholesterol decanoate + H2O 34% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + decanoic acid
-
 ?
cholesterol hexanoate + H2O 29% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + hexanoic acid
-
 ?
cholesterol linoleate + H2O
-
 Burkholderia cepacia cholesterol + linoleic acid
-
 ?
cholesterol oleate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + oleic acid
-
 ?
cholesterol oleate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + oleic acid
-
 ?
cholesterol palmitate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + palmitic acid
-
 ?
p-nitrophenyl acetate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 p-nitrophenol + acetate
-
 ?
p-nitrophenyl hexanoate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + hexanoic acid
-
 ?
p-nitrophenyl laurate + H2O 92% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + lauric acid
-
 ?
p-nitrophenyl myristate + H2O 100% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + myristic acid
-
 ?
p-nitrophenyl octanoate + H2O 79% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + octanoic acid
-
 ?
p-nitrophenyl palmitate + H2O 68% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + palmitic acid
-
 ?
p-nitrophenyl propionate + H2O 17% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + propionic acid
-
 ?
p-nitrophenyl stearate + H2O 38% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + stearic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 65
-
 Burkholderia cepacia
75
-
 almost complete loss of activity Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Burkholderia cepacia

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5
-
 Burkholderia cepacia

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 12
-
 Burkholderia cepacia