Application | Comment | Organism |
---|---|---|
analysis | immobilization of the enzyme alone or coimmobilization with cholesterol oxidase on zirkonia-coated acrylamine glass beads for analysis of total cholesterol in serum, long-term stability, precise measurements with the enzyme mixture at cholesterol concentration above 1 g/l | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
pancreas | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
4°C, in distilled water, 3 months during regular use in analysis, 30% loss of activity for the free enzyme, 17% loss of activity for the immobilized enzyme, 18% loss of activity for the coimmobilized enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl acetate + H2O | - |
Bos taurus | cholesterol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cholesterol esterase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
coimmobilized enzyme | Bos taurus |
37 | - |
assay at | Bos taurus |
40 | - |
optimum, immobilized and free enzyme | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
30 min, 18% remaining activity for the free enzyme, 43% remaining activity for the immobilized enzyme, 48% remaining activity for the coimmobilized enzyme | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
free enzyme, coimmobilized enzyme | Bos taurus |
7.5 | - |
immobilized enzyme | Bos taurus |