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Literature summary for 3.1.1.13 extracted from

  • Stoddard Hatch, M.; Brown, W.M.; Deck, J.A.; Hunsaker, L.A.; Deck, L.M.; Vander Jagt, D.L.
    Inhibition of yeast lipase (CRL1) and cholesterol esterase (CRL3) by 6-chloro-2-pyrones: comparison with porcine cholesterol esterase (2002), Biochim. Biophys. Acta, 1596, 381-391.
    View publication on PubMed
Search for more literature for 3.1.1.13

Activating Compound

Activating Compound Comment Organism Structure
taurocholate activates CRL3, not CRL1 Limtongozyma cylindracea

Inhibitors

Inhibitors Comment Organism Structure
6-chloro-3-(2-cyclohexylethyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(2-cyclohexylethyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(2-cyclopentylethyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(2-cyclopentylethyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(2-phenylethyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(2-phenylethyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(3-cyclohexylpropyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(3-cyclohexylpropyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(4-cyclohexylbutyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(4-cyclohexylbutyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-(cyclohexylmethyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-(cyclohexylmethyl)-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-cyclohexyl-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-cyclohexyl-2H-pyran-2-one
-
 Sus scrofa
6-chloro-3-cyclopentyl-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-3-cyclopentyl-2H-pyran-2-one
-
 Sus scrofa
6-chloro-5-(2-cyclohexylethyl)-3-methyl-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-5-(2-cyclohexylethyl)-3-methyl-2H-pyran-2-one
-
 Sus scrofa
6-chloro-5-methyl-3-(2-phenylethyl)-2H-pyran-2-one competitive Limtongozyma cylindracea
6-chloro-5-methyl-3-(2-phenylethyl)-2H-pyran-2-one
-
 Sus scrofa
taurocholate inhibits CRL1, not CRL3 Limtongozyma cylindracea
taurocholate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 4-nitrophenyl butyrate CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.07
-
 4-nitrophenyl butyrate CRL3, pH 7.4, 25°C, in presence of taurocholate Limtongozyma cylindracea
0.206
-
 4-nitrophenyl butyrate CRL1, pH 7.4, 25°C, in presence of taurocholate Limtongozyma cylindracea
0.219
-
 4-nitrophenyl butyrate CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.37
-
 4-nitrophenyl butyrate pH 7.4, 25°C, in presence of taurocholate Sus scrofa
0.73
-
 4-nitrophenyl butyrate pH 7.4, 25°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Limtongozyma cylindracea
-
 enzyme forms CRL1 and CRL3
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
 Sus scrofa 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Limtongozyma cylindracea 4-nitrophenol + butyrate
-
 ?
additional information CRL1 slowly hydrolyzes 6-chloro-2-pyrones Limtongozyma cylindracea ?
-
 ?

Synonyms

Synonyms Comment Organism
CEase
-
 Sus scrofa
CEase
-
 Limtongozyma cylindracea
cholesterol esterase
-
 Sus scrofa
cholesterol esterase
-
 Limtongozyma cylindracea
CRL1
-
 Limtongozyma cylindracea
CRL3
-
 Limtongozyma cylindracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sus scrofa
25
-
 assay at Limtongozyma cylindracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
 4-nitrophenyl butyrate CRL3, pH 7.4, 25°C Limtongozyma cylindracea
11.8
-
 4-nitrophenyl butyrate CRL3, pH 7.4, 25°C, in presence of taurocholate Limtongozyma cylindracea
400
-
 4-nitrophenyl butyrate pH 7.4, 25°C, in presence of taurocholate Sus scrofa
650
-
 4-nitrophenyl butyrate pH 7.4, 25°C Sus scrofa
60000
-
 4-nitrophenyl butyrate CRL1, pH 7.4, 25°C, in presence of taurocholate Limtongozyma cylindracea
63300
-
 4-nitrophenyl butyrate CRL1, pH 7.4, 25°C Limtongozyma cylindracea
63330
-
 4-nitrophenyl butyrate CRL1, pH 7.4, 25°C Limtongozyma cylindracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Sus scrofa
7.4
-
 assay at Limtongozyma cylindracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000025
-
 6-chloro-3-(2-cyclohexylethyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00004
-
 6-chloro-3-cyclopentyl-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.000058
-
 6-chloro-5-(2-cyclohexylethyl)-3-methyl-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.000063
-
 6-chloro-3-(3-cyclohexylpropyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00013
-
 6-chloro-3-(2-phenylethyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00021
-
 6-chloro-3-(4-cyclohexylbutyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.00023
-
 6-chloro-3-(3-cyclohexylpropyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.0005
-
 6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00051
-
 6-chloro-5-methyl-3-(2-phenylethyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00058
-
 6-chloro-3-(4-cyclohexylbutyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.00073
-
 6-chloro-3-(2-cyclohexylethyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.0008
-
 6-chloro-3-(2-cyclopentylethyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.0022
-
 6-chloro-3-(cyclohexylmethyl)-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.0023
-
 6-chloro-3-cyclohexyl-2H-pyran-2-one pH 7.4, 25°C Sus scrofa
0.0044
-
 6-chloro-3-(4-cyclohexylbutyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.011
-
 6-chloro-3-(3-cyclohexylpropyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.012
-
 6-chloro-3-(cyclohexylmethyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.013
-
 6-chloro-5-(2-cyclohexylethyl)-3-methyl-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.016
-
 6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.045
-
 6-chloro-3-(2-cyclopentylethyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.053
-
 6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.058
-
 6-chloro-3-cyclopentyl-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.084
-
 6-chloro-3-cyclohexyl-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.096
-
 6-chloro-3-(2-phenylethyl)-2H-pyran-2-one CRL3, pH 7.4, 25°C Limtongozyma cylindracea
0.11
-
 6-chloro-3-(2-cyclohexylethyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.11
-
 6-chloro-3-cyclohexyl-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.12
-
 6-chloro-3-(cyclohexylmethyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.12
-
 6-chloro-3-(2-phenylethyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.16
-
 6-chloro-5-(2-cyclohexylethyl)-3-methyl-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.225
-
 6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.24
-
 6-chloro-3-(2-cyclopentylethyl)-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.9
-
 6-chloro-3-(2-cyclopentylethyl)-5-methyl-2H-pyran-2-one above, CRL1, pH 7.4, 25°C Limtongozyma cylindracea
0.96
-
 6-chloro-3-cyclopentyl-2H-pyran-2-one CRL1, pH 7.4, 25°C Limtongozyma cylindracea