Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase type II | activation only for neutral CEase | Oryctolagus cuniculus | |
dibutyryl cAMP | 2fold stimulation | Oryctolagus cuniculus | |
glucagon | 2fold stimulation for neutral CEase | Oryctolagus cuniculus | |
sodium taurocholic acid | - |
Oryctolagus cuniculus |
General Stability | Organism |
---|---|
neutral CEase stable to repeated freezing and thawing, acid CEase unstable | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium taurocholic acid | marked inhibition above 0.015 mM | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
cholesteryl oleate | for neutral CEase | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
lipid particle | cholesteryl ester rich droplet | Oryctolagus cuniculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | Oryctolagus cuniculus | important rate-limiting step in steroidogenesis | cholesterol + fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
rabbit | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
for neutral CEase in presence of dibutyryl-cAMP, MgATP2- and cAMP-dependent protein kinase | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-70°C, neutral CEase, 1 month. | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | important rate-limiting step in steroidogenesis | Oryctolagus cuniculus | cholesterol + fatty acid | - |
? | |
cholesteryl oleate + H2O | - |
Oryctolagus cuniculus | cholesterol + oleic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid cholesterol esterase | - |
Oryctolagus cuniculus |
neutral cholesterol esterase | - |
Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
80% loss of activity for acid CEase and 25% for neutral CEase at pH 7.0 | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cAMP | activation only for neutral CEase | Oryctolagus cuniculus | |
cAMP | as cofactor for cAMP-dependent protein kinase | Oryctolagus cuniculus | |
MgATP2- | activation only for neutral CEase | Oryctolagus cuniculus | |
MgATP2- | increasing activity with addition of cAMP-dependend protein kinase | Oryctolagus cuniculus |