Activating Compound | Comment | Organism | Structure |
---|---|---|---|
follicle-stimulating hormone | 26fold activation after 14 days | Rattus sp. | |
luteinizing hormone | stimualtes only temperature-stable enzyme | Rattus sp. | |
sodium cholate | - |
Sus scrofa | |
sodium cholate | maximal activation at 0.03-0.05% v/v | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholesteryl esters | inhibition at 0.2 mM with cholesteryl-stearate, -oleate, -eicosenoate, -arachidonate, -erucate and -nervonate | Rattus sp. | |
sodium cholate | 50% inhibition at 0.05-0.1% v/v | Rattus sp. | |
sodium deoxycholate | complete inhibition | Rattus sp. | |
Sodium dodecyl sulfate | complete inactivation | Rattus sp. | |
Triton X-100 | complete inactivation | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel permeation, temperature-stable enzyme | Rattus sp. |
72000 | - |
gel filtration, temperature-labile enzyme | Rattus sp. |
420000 | - |
gel filtration, temperature-labile enzyme | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl esters + H2O | Sus scrofa | - |
cholesterol + fatty acid | - |
? | |
cholesteryl esters + H2O | Rattus sp. | involvement in inhibition of spermatogenesis | cholesterol + fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by HPLC separation of temperature-labile and temperature-stable enzymes | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus sp. | - |
Leydig cell | temperature-stabile enzyme enriched | Rattus sp. | - |
liver | - |
Rattus sp. | - |
pancreas | - |
Sus scrofa | - |
pancreas | - |
Rattus sp. | - |
Sertoli cell | temperature-labile enzyme enriched | Rattus sp. | - |
testis | temperature-labile enzyme exclusively localized | Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl arachidonate + H2O | - |
Sus scrofa | cholesterol + arachidonic acid | - |
? | |
cholesteryl arachidonate + H2O | - |
Rattus sp. | cholesterol + arachidonic acid | - |
? | |
cholesteryl eicosenoate + H2O | - |
Sus scrofa | cholesterol + eicosenoic acid | - |
? | |
cholesteryl eicosenoate + H2O | - |
Rattus sp. | cholesterol + eicosenoic acid | - |
? | |
cholesteryl erucate + H2O | - |
Sus scrofa | cholesterol + erucic acid | 13-docosenoic acid | ? | |
cholesteryl erucate + H2O | - |
Rattus sp. | cholesterol + erucic acid | 13-docosenoic acid | ? | |
cholesteryl esters + H2O | - |
Sus scrofa | cholesterol + fatty acid | - |
? | |
cholesteryl esters + H2O | involvement in inhibition of spermatogenesis | Rattus sp. | cholesterol + fatty acid | - |
? | |
cholesteryl nervonate + H2O | - |
Sus scrofa | cholesterol + nervonic acid | - |
? | |
cholesteryl nervonate + H2O | especially for temperature-labile cholesteryl esterase | Rattus sp. | cholesterol + nervonic acid | - |
? | |
cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
? | |
cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
? | |
cholesteryl stearate + H2O | - |
Sus scrofa | cholesterol + stearic acid | - |
? | |
cholesteryl stearate + H2O | - |
Rattus sp. | cholesterol + stearic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
dimer | 1 * 28000 SDS-PAGE, gel filtration (HPLC gel permeation) | Rattus sp. |
multimer | - |
Sus scrofa |
multimer | multimeric monomers of 72000 each, SDS-PAGE, gel filtration (HPLC gel permeation) temperature-labile enzyme | Rattus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
temperature-labile enzyme | Rattus sp. |
37 | - |
temperature-stable enzyme | Rattus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
inactivation by evaluation from 32°C to 37°C | Rattus sp. |