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Literature summary for 3.1.1.13 extracted from
- Porcine cholesterol esterase, a multiform enzyme (1983), Biochim. Biophys. Acta, 749, 32-41.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Sus scrofa | - |
- |
| intracellular | - |
Sus scrofa | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration (HPLC separation) | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | Sus scrofa | - |
cholesterol + fatty acid | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein, glucosamine, no galactosamine, neutral sugars | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by digitonin solubilization, chromatography steps, isoelectric focusing, isocratic HPLC | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
for cholesteryl oleate in monomeric enzyme | Sus scrofa |
| 0.19 | - |
for cholesteryl oleate in dimeric enzyme | Sus scrofa |
| 16.43 | - |
for p-nitrophenyl acetate in monomeric enzyme | Sus scrofa |
| 25.93 | - |
for p-nitrophenyl acetate in dimeric enzyme | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesteryl esters + H2O | - |
Sus scrofa | cholesterol + fatty acid | - |
? | |
| cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Sus scrofa | p-nitrophenol + acetic acid | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 45000 gel filtration (HPLC separation) | Sus scrofa |
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