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Literature summary for 3.1.1.13 extracted from

  • Tuhackovaá, Z.; Kriz, O.; Hradec, J.
    Purification and some properties of a cholesterol esterase from rat liver (1980), Biochim. Biophys. Acta, 617, 439-445.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium taurocholic acid absolutely required, glycocholate can fully be substituted Rattus sp.
Trihydroxy bile salts lead to conversion of enzyme tetramers into monomers Rattus sp.

General Stability

General Stability Organism
freezing and storage at -80°C deactivate, addition of bovine serum albumin increases activity Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
deoxycholate 0.1% v/v, hydrolysis not detectable Rattus sp.
N-ethylmaleimide complete loss of activity Rattus sp.
Triton X-100 0.1% v/v, 12% substrate hydrolyzed in the presence of bile salts Rattus sp.
Tween 40 0.1% v/v, hydrolysis not detectable Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
cholesteryl oleate
-
Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Rattus sp.
Mg2+
-
Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesteryl esters + H2O Rattus sp.
-
cholesterol + fatty acid
-
ir

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone reduces hydrolysis rate significantly Rattus sp.
Glycerol inactivates Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3step chromatography techniques, preparative electrophoresis to homogeneity Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
-
Rattus sp.

Storage Stability

Storage Stability Organism
0°C, 1 week Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesteryl esters + H2O
-
Rattus sp. cholesterol + fatty acid
-
ir
cholesteryl oleate + H2O
-
Rattus sp. cholesterol + oleic acid
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 65000, native PAGE, SDS-PAGE, activity also in monomers Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Rattus sp.