Literature summary for 3.1.1.13 extracted from

Mori, S.; Nakata,Y.; Endo, H.
Enzymatic properties of polyethylene glycol-modified cholesterol esterase in organic solvents (1992), Biotechnol. Appl. Biochem., 16, 101-105.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cholesteryl esters + H2O	Pseudomonas sp.	long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids	cholesterol + fatty acid	-	r

Organic Solvent Stability

Organic Solvent	Comment	Organism
chloroform	most suitable for cholesteryl linoleate synthesis in polyethylene glycol-modified enzyme	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesteryl esters + H2O	long-chain fatty acid cholesterol esters more easily synthesized, synthetic activity highest in chloroform, activity enhanced in degree of unsaturation of fatty acids	Pseudomonas sp.	cholesterol + fatty acid	-	r
cholesteryl laurate + H2O	-	Pseudomonas sp.	cholesterol + lauric acid	-	r
cholesteryl linoleate + H2O	synthesis of cholesterol linoleate from polyethylene glycol-modified enzyme only in water-immiscible solvents	Pseudomonas sp.	cholesterol + linoleic acid	-	r
cholesteryl myristate + H2O	-	Pseudomonas sp.	cholesterol + myristic acid	-	r
cholesteryl oleate + H2O	-	Pseudomonas sp.	cholesterol + oleic acid	-	r
cholesteryl palmitate + H2O	-	Pseudomonas sp.	cholesterol + palmitic acid	-	r
cholesteryl stearate + H2O	-	Pseudomonas sp.	cholesterol + stearic acid	-	r

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Release 2023.1 (January 2023)