Cloned (Comment) | Organism |
---|---|
gene PME6, DNA and amino acid sequence determination and analysis, recombinant expression of YFP-tagged enzyme in the seed coat and cells of the embryos, the HMS-YFP fusions does not have enough HMS activity to compensate for the hms mutation | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a hms-1 mutant from gene PME6 by a transposon insertion. The hms-1 embryo defect phenotype is caused by a mutation in HMS, molecular complementation is performed, phenotype overview. Analysis of a second hms allele, allele gabi-kat-278G11, i.e. hms-2. The T-DNA in hms-2 is inserted at 229 from the +1 site in the 5' UTR. The hms-2 mutant has no mucilage phenotype, and HMS transcript in hms-2 is comparable with the wild-type. No other alleles are available in the coding region of HMS | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | endogenous inhibitor PMEI5 does not act through HMS in the embryo cell wall | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + n H2O | Arabidopsis thaliana | - |
n methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O49298 | ecotypes Nos-0 and Col-0 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | analysis of seed anatomy and embryo cell sizes throughout development of wild-type and hms-1 mutant | Arabidopsis thaliana | - |
plant embryo | - |
Arabidopsis thaliana | - |
seed coat | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + n H2O | - |
Arabidopsis thaliana | n methanol + pectate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At1g23200 | locus name | Arabidopsis thaliana |
HMS | - |
Arabidopsis thaliana |
hms-1 | - |
Arabidopsis thaliana |
pectin methyl esterase | - |
Arabidopsis thaliana |
PME6 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | a defect in mucilage extrusion is observed in a PME6 mutant and is shown to be a pleiotropic effect of the changes in embryo. hms-1 Embryo defect phenotype, the embryo cell size is decreased, the hms-1 radicals and cotyledons both have a reduced cell perimeter compared with the wild-type, overview. The PME activity is decreased and the degree of methyl esterification is increased in hms-1 7-DPA mutant seeds | Arabidopsis thaliana |
physiological function | highly methyl esterified seeds' (gene PME6) is a pectin methyl esterase involved in embryo development, it is required for normal embryo development. Enzyme HMS causes the softening of plant tissues. Isozyme HMS plays an important role in embryo growth | Arabidopsis thaliana |