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Literature summary for 3.1.1.11 extracted from
- Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937 (1996), Mol. Microbiol., 19, 455-466.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Dickeya chrysanthemi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 0.1% complete inactivation | Dickeya chrysanthemi |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Dickeya chrysanthemi | the enzyme is required for the growth of bacteria on oligomeric substrates, probably involved in the degradation of methylated oligogalacturonides present in the periplasm of the bacteria | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dickeya chrysanthemi | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | lipoprotein | Dickeya chrysanthemi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methylated oligogalacturonides + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| pectin + H2O | - |
Dickeya chrysanthemi | methanol + pectate | - |
? | |
| pectin + H2O | the enzyme is required for the growth of bacteria on oligomeric substrates, probably involved in the degradation of methylated oligogalacturonides present in the periplasm of the bacteria | Dickeya chrysanthemi | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Dickeya chrysanthemi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
5 h, stable | Dickeya chrysanthemi |
| 50 | - |
half-life: 10 min | Dickeya chrysanthemi |
| 60 | - |
complete loss of activity after 5 min | Dickeya chrysanthemi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Dickeya chrysanthemi |
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