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Literature summary for 3.1.1.106 extracted from

  • Haikarainen, T.; Maksimainen, M.M.; Obaji, E.; Lehtioe, L.
    Development of an inhibitor screening assay for mono-ADP-ribosyl hydrolyzing macrodomains using AlphaScreen technology (2018), SLAS Discov., 23, 255-263 .
    View publication on PubMed
Search for more literature for 3.1.1.106

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP-D-ribose
-
 Homo sapiens
Ethacrynic acid
-
 Homo sapiens
patulin most potent inhibitor Homo sapiens
thimerosal
-
 Homo sapiens
triclosan
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
 ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O Homo sapiens
-
 ADP-D-ribose + acetate
-
 ?
[SRPK2 protein]-ADP-D-ribose + H2O Homo sapiens
-
 [SRPK2 protein] + ADP-D-ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BQ69
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2''-O-acetyl-ADP-D-ribose + H2O
-
 Homo sapiens ADP-D-ribose + acetate
-
 ?
3''-O-acetyl-ADP-D-ribose + H2O
-
 Homo sapiens ADP-D-ribose + acetate
-
 ?
[SRPK2 protein]-ADP-D-ribose + H2O
-
 Homo sapiens [SRPK2 protein] + ADP-D-ribose
-
 ?

Synonyms

Synonyms Comment Organism
MACROD1
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0025
-
 at pH 7.5 and 25°C Homo sapiens patulin
0.0052
-
 at pH 7.5 and 25°C Homo sapiens thimerosal
0.027
-
 at pH 7.5 and 25°C Homo sapiens Ethacrynic acid
0.1
-
 at pH 7.5 and 25°C Homo sapiens triclosan