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Literature summary for 3.1.1.1 extracted from

  • Fucinos, P.; Atanes, E.; Lopez-Lopez, O.; Solaroli, M.; Cerdan, M.; Gonzalez-Siso, M.; Pastrana, L.; Rua, M.
    Cloning, expression, purification and characterization of an oligomeric His-tagged thermophilic esterase from Thermus thermophilus HB27 (2014), Process Biochem., 49, 927-935 .
No PubMed abstract available

Application

Application Comment Organism
industry the enzyme is highly thermostable (half-life of 107.9 min at 85°C), suggesting that E34Tt-His6 has potential for industrial applications Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged protein Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71800
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72J75
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl decanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + decanoate
-
?
4-nitrophenyl dodecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl hexadecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + hexadecanoate
-
?
4-nitrophenyl tetradecanoate + H2O 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate Thermus thermophilus 4-nitrophenol + tetradecanoate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37700, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
E34Tt
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
half-life: 107.9 min Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
His6-tagged recombinant enzyme Thermus thermophilus
8.5
-
wild-type enzyme Thermus thermophilus