Application | Comment | Organism |
---|---|---|
industry | the enzyme is highly thermostable (half-life of 107.9 min at 85°C), suggesting that E34Tt-His6 has potential for industrial applications | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged protein | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71800 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72J75 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl decanoate + H2O | 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate | Thermus thermophilus | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate | Thermus thermophilus | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexadecanoate + H2O | 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate | Thermus thermophilus | 4-nitrophenol + hexadecanoate | - |
? | |
4-nitrophenyl tetradecanoate + H2O | 4-nitrophenyl decanoate is the most readily hydrolyzed substrate. The activity decreases sharply with increasing the chain length, being 4-nitrophenyldecanoate hydrolyzed at five times the rate of 4-nitrophenyl hexadecanoate | Thermus thermophilus | 4-nitrophenol + tetradecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37700, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
E34Tt | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life: 107.9 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
His6-tagged recombinant enzyme | Thermus thermophilus |
8.5 | - |
wild-type enzyme | Thermus thermophilus |