Literature summary for 3.1.1.1 extracted from

Rao, L.; Zhao, X.; Pan, F.; Li, Y.; Xue, Y.; Ma, Y.; Lu, J.R.
Solution behavior and activity of a halophilic esterase under high salt concentration (2009), PLoS One, 4, e6980.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed from Escherichia coli BL21	Haloarcula marismortui

General Stability

General Stability	Organism
influence of NaCl on the stability of the enzyme is highly temperature dependent. At 22°C, the enzyme retains its highest activity when incubated at the high salt concentration of 3.4 M NaCl for over 100 hours. In contrast, the enzyme steadily loses its activity without salt, with most of its activity being lost within 50 h. As the incubation temperature is raised to 37°C, the activities under both solution conditions show a clear sign of decline, although the presence of 3.4 M NaCl sustains the activity more effectively. When the incubation temperature reaches 55°C, the rates of activity loss are significantly increased with and without the addition of 3.4 M NaCl. With further increase in temperature to 60°C the trend of acceleration of activity loss continues and salt addition again accelerates the rate of denaturation, confirming that whilst salt addition helps stabilize the enzyme below 50°C, the opposite effect is produced above it	Haloarcula marismortui

Organism

influence of NaCl on the stability of the enzyme is highly temperature dependent. At 22°C, the enzyme retains its highest activity when incubated at the high salt concentration of 3.4 M NaCl for over 100 hours. In contrast, the enzyme steadily loses its activity without salt, with most of its activity being lost within 50 h. As the incubation temperature is raised to 37°C, the activities under both solution conditions show a clear sign of decline, although the presence of 3.4 M NaCl sustains the activity more effectively. When the incubation temperature reaches 55°C, the rates of activity loss are significantly increased with and without the addition of 3.4 M NaCl. With further increase in temperature to 60°C the trend of acceleration of activity loss continues and salt addition again accelerates the rate of denaturation, confirming that whilst salt addition helps stabilize the enzyme below 50°C, the opposite effect is produced above it

Haloarcula marismortui

Metals/Ions

Metals/Ions	Comment	Organism	Structure
KCl	optimal concentration: 3.0 M	Haloarcula marismortui
NaCl	optimal concentration: 3.4 M	Haloarcula marismortui

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	x * 50000, SDS-PAGE	Haloarcula marismortui

Organism

Organism	UniProt	Comment	Textmining
Haloarcula marismortui	Q5V5N6	-	-
Haloarcula marismortui DSM 3752	Q5V5N6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Haloarcula marismortui

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-nitrophenyl acetate + H2O	-	Haloarcula marismortui	4-nitrophenol + acetate	-	?
4-nitrophenyl acetate + H2O	-	Haloarcula marismortui DSM 3752	4-nitrophenol + acetate	-	?
4-nitrophenyl butyrate + H2O	about 60% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui	4-nitrophenol + butyrate	-	?
4-nitrophenyl butyrate + H2O	about 60% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui DSM 3752	4-nitrophenol + butyrate	-	?
4-nitrophenyl hexanoate + H2O	about 30% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui	4-nitrophenol + hexanoate	-	?
4-nitrophenyl hexanoate + H2O	about 30% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui DSM 3752	4-nitrophenol + hexanoate	-	?
4-nitrophenyl octanoate + H2O	about 5% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui	4-nitrophenol + octanoate	-	?
4-nitrophenyl octanoate + H2O	about 5% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui DSM 3752	4-nitrophenol + octanoate	-	?
4-nitrophenyl propanoate + H2O	about 95% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui	4-nitrophenol + propanoate	-	?
4-nitrophenyl propanoate + H2O	about 95% of the activity with 4-nitrophenyl acetate	Haloarcula marismortui DSM 3752	4-nitrophenol + propanoate	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE	Haloarcula marismortui

Synonyms

Synonyms	Comment	Organism
LipC	-	Haloarcula marismortui

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Haloarcula marismortui

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	55	40°C: about 65% of maximal activity, 55°C: about 75% of maximal activity	Haloarcula marismortui

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	whilst salt addition helps stabilize the enzyme below 50°C, the opposite effect is produced above it	Haloarcula marismortui

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	-	Haloarcula marismortui

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	10	pH 8.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity	Haloarcula marismortui