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Literature summary for 3.1.1.1 extracted from

  • Kim, S.; Lee, S.B.
    Thermostable esterase from a thermoacidophilic archaeon: purification and characterization for enzymatic resolution of a chiral compound (2004), Biosci. Biotechnol. Biochem., 68, 2289-2298.
    View publication on PubMed
Search for more literature for 3.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
ethanol 5% (w/v), 1.1fold activation Saccharolobus solfataricus
Tween 60 5% (w/v), 1.6fold activation Saccharolobus solfataricus

Application

Application Comment Organism
synthesis synthesis of chiral compounds Saccharolobus solfataricus
synthesis the enzyme can be used for application in the synthesis of chiral compounds Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus
expression in Escherichia coli as a 6His-tagged fusion protein Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
1-butanol 5% (v/v), 95% inhibition; 5% (w/v), 95% inhibition Saccharolobus solfataricus
1-propanol 5% (v/v), 91% inhibition; 5% (w/v), 91% inhibition Saccharolobus solfataricus
2-butanol 10% (v/v), 52% inhibition; 10% (w/v), 42% inhibition Saccharolobus solfataricus
2-mercaptoethanol 1 mM, 8% inhibition Saccharolobus solfataricus
2-propanol 10% (v/v), 94% inhibition; 5% (w/v), 58% inhibition. 10% (w/v), 94% inhibition Saccharolobus solfataricus
acetone 10% (v/v), 88% inhibition; 10% (w/v), 88% inhibition Saccharolobus solfataricus
acetonitrile 10% (w/v), 61% inhibition; 5% (v/v), 61% inhibition Saccharolobus solfataricus
diethyl 4-nitrophenyl phosphate 1 mM, 70% inhibition Saccharolobus solfataricus
diethyl dicarbonate 1 mM, 19% inhibition Saccharolobus solfataricus
Diethyl p-nitrophenyl phosphate 1 mM, 70% inhibition Saccharolobus solfataricus
DMSO 10% (w/v), 66% inhibition; 5% (v/v), 58% inhibition Saccharolobus solfataricus
eserine 1 mM, 12% inhibition Saccharolobus solfataricus
HgCl2 1 mM, 53% inhibition Saccharolobus solfataricus
methanol 10% (v/v), 93% inhibition; 5% (w/v), 94% inhibition Saccharolobus solfataricus
additional information no inhibition by EDTA Saccharolobus solfataricus
phenylmethylsulfonyl fluoride 1 mM, 72% inhibition Saccharolobus solfataricus
Triton X-100 10% (v/v), 92% inhibition; 10% (w/v), 92% inhibition Saccharolobus solfataricus
Tween 5% (v/v), 94% inhibition Saccharolobus solfataricus
Tween 20 5% (w/v), 94% inhibition Saccharolobus solfataricus
Tween 50 10% (v/v), 49% inhibition Saccharolobus solfataricus
Tween 60 10% (w/v), 49% inhibition Saccharolobus solfataricus
Tween 80 5% (w/v), 8% inhibition Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
2.1
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus
2.5
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
2.5
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
4.8
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
4.8
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
14.5
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus
14.5
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
67.5
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
67.6
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Saccharolobus solfataricus 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000 Saccharolobus solfataricus
32000
-
 3 * 32000 Saccharolobus solfataricus
33428
-
 3 * 33428, calculated from sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VW1
-
-
Saccharolobus solfataricus P2 Q97VW1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus
recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3133
-
 70°C, pH 7.2 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-(3-benzoylphenyl)propanoic methyl ester + H2O when tested with the racemic substrate, the enzyme leads to the production of the R-configured acid with an enantiomeric excess of 80% at the conversion rate of 20% in 32 h Saccharolobus solfataricus methanol + (R)-2-(3-benzoylphenyl)propanoate
-
 ?
4-nitrophenyl acetate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O hydrolysis at about 70% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O hydrolysis at about 70% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O hydrolysis at about 90% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl decanoate + H2O hydrolysis at about 50% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl dodecanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl dodecanoate + H2O hydrolysis at about 30% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexadecanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + hexadecanoate
-
 ?
4-nitrophenyl hexadecanoate + H2O hydrolysis at less than 10% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + hexadecanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O hydrolysis at about 90% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl octanoate + H2O hydrolysis at about 75% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + pentanoate
-
 ?
4-nitrophenyl propanoate + H2O hydrolysis at about 85% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + propanoate
-
 ?
4-nitrophenyl tetradecanoate + H2O hydrolysis at less tha 10% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + tetradecanoate
-
 ?
methyl (-)-2-(3-benzoylphenyl)propionate + H2O
-
 Saccharolobus solfataricus (-)-2-(3-benzoylphenyl)propionate + methanol
-
 ?
additional information broad substrate specificities toward various p-nitrophenyl esters ranging from C2 to C16. The activity of the enzyme decreases slowly from 4-nitrophenyl hexanoate to 4-nitrophenyl dodecanoate and is very low for 4-nitrophenyl 4-nitrophenyl hexadecanoate Saccharolobus solfataricus ?
-
 ?
additional information broad substrate specificities toward various p-nitrophenyl esters ranging from C2 to C16. The activity of the enzyme decreases slowly from 4-nitrophenyl hexanoate to 4-nitrophenyl dodecanoate and is very low for 4-nitrophenyl 4-nitrophenyl hexadecanoate Saccharolobus solfataricus P2 ?
-
 ?

Subunits

Subunits Comment Organism
trimer x * 32000 Saccharolobus solfataricus
trimer 3 * 32000 Saccharolobus solfataricus
trimer 3 * 33428, calculated from sequence Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
ESt3
-
 Saccharolobus solfataricus
Est3 esterase
-
 Saccharolobus solfataricus
SSO2493 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
80
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 40°C: about 50% of maximal activity, 90°C: about 70% of maximal activity Saccharolobus solfataricus
60 90 60°C: about 60% of maximal activity, 90°C: about 70% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 2 h, less than 10% loss of activity Saccharolobus solfataricus
70
-
 2 h, less than 10% loss of activity Saccharolobus solfataricus
80
-
 half-life: 40 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.8
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
45.3
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
45.3
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
46.3
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus
46.3
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
78
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus
78
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
79.3
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
79.3
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharolobus solfataricus
7.4
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 40% of maximal activity, pH 10.0: about 50% of maximal activity Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.7
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
0.7
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
1.2
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
1.2
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus
5.1
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus
5.1
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
16.2
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
16.2
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
21.1
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
21.1
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus