Literature summary for 3.1.1.1 extracted from

Hemmert, A.C.; Otto, T.C.; Wierdl, M.; Edwards, C.C.; Fleming, C.D.; MacDonald, M.; Cashman, J.R.; Potter, P.M.; Cerasoli, D.M.; Redinbo, M.R.
Human carboxylesterase 1 stereoselectively binds the nerve agent cyclosarin and spontaneously hydrolyzes the nerve agent sarin (2010), Mol. Pharmacol., 77, 508-516.

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Activating Compound

Activating Compound	Comment	Organism	Structure
sarin	for enzyme inhibited by racemic mixtures of bona fide nerve agents, hCE1 spontaneously reactivates in the presence of sarin but not soman or cyclosarin. The addition of 2,3-butanedione monoxime increases the rate of reactivation of CE1 from sarin inhibition by more than 65fold	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in baculovirus-infected Spodoptera frugiperda Sf21 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
carboxylesterase 1 in complex with cyclosarin, sitting drop vapor diffusion method, using 10% (w/v) polyethylene glycol 3350, 0.3 M Li2SO4, 0.1 M citrate, pH 5.5, 0.1 M NaCl, 0.1 M LiCl, and 5% (v/v) glycerol.	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cyclosarin	-	Homo sapiens
additional information	2,3-butanedione monoxime has no inhibitory effect on CE1	Homo sapiens
soman	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl butyrate + H2O	-	Homo sapiens	4-nitrophenol + butyrate	-	?
additional information	human carboxylesterase 1 stereoselectively binds the nerve agent cyclosarin and exhibits a 1700- and 2900fold preference for the PR enantiomers of analogs of soman and cyclosarin, respectively	Homo sapiens	?	-	?
sarin + H2O	CE1 exhibits a 5fold preference for the PS isomer of a sarin analog	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
carboxylesterase 1	-	Homo sapiens
CE1	-	Homo sapiens

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Release 2023.1 (January 2023)