Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli JM105 cells | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
C60A | the mutant shows higher affinity to the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme | Arthrobacter globiformis |
K62E | inactive with (+)-trans-ethyl crysanthemate | Arthrobacter globiformis |
K62R | the mutant shows 7% turnover rate with the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme | Arthrobacter globiformis |
KK62L | inactive with (+)-trans-ethyl crysanthemate | Arthrobacter globiformis |
S53A | the mutant has about wild type affinity to the substrate (+)-trans-ethyl crysanthemate | Arthrobacter globiformis |
S59A | inactive with (+)-trans-ethyl crysanthemate | Arthrobacter globiformis |
S61A | the mutant shows higher affinity to the substrate (+)-trans-ethyl crysanthemate compared to the wild type enzyme | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
(+)-trans-ethyl crysanthemate | mutant enzyme S61A, pH 7.5, 45°C | Arthrobacter globiformis | |
0.18 | - |
(+)-trans-ethyl crysanthemate | mutant enzyme C60A, pH 7.5, 45°C | Arthrobacter globiformis | |
0.31 | - |
(+)-trans-ethyl crysanthemate | mutant enzyme S53A, pH 7.5, 45°C | Arthrobacter globiformis | |
0.33 | - |
(+)-trans-ethyl crysanthemate | wild type enzyme, pH 7.5, 45°C | Arthrobacter globiformis | |
10 | - |
(+)-trans-ethyl crysanthemate | mutant enzyme K62R, pH 7.5, 45°C | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-5PW column chromatography | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-trans 4-nitrophenyl crysanthemate + H2O | - |
Arthrobacter globiformis | (+)-trans-crysanthemic acid + 4-nitrophenol | - |
? | |
(+)-trans-ethyl crysanthemate + H2O | - |
Arthrobacter globiformis | (+)-trans-crysanthemic acid + ethanol | - |
? | |
phenolphthalein dibutyrate + H2O | - |
Arthrobacter globiformis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
wild type enzyme | Arthrobacter globiformis |