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Literature summary for 3.1.1.1 extracted from
- Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01 (2010), Appl. Biochem. Biotechnol., 162, 1574-1584.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (S)-naproxen | nearly 80% of the initial activity is lost when 2.5 mM of (S)-naproxen is added to the reaction system | Bacillus subtilis |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | D0EPY0 | - |
- |
| Bacillus subtilis ECU0554 | D0EPY0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-naproxen methyl ester + H2O | - |
Bacillus subtilis | (S)-naproxen + methanol | i.e. (+)-(S)-2-(6-methoxy-2-naphthyl)propionic acid, 98% enantiomeric excess | ? | |
| 4-nitrophenyl acetate + H2O | about 30% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | about 30% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis ECU0554 | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | highest activity (100%) | Bacillus subtilis | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | highest activity (100%) | Bacillus subtilis ECU0554 | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caproate + H2O | about 55% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl decanoate + H2O | about 30% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl dodecanoate + H2O | about 20% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + dodecanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | about 45% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl octanoate + H2O | about 45% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis ECU0554 | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl propionate + H2O | about 70% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis | 4-nitrophenol + propionate | - |
? | |
| 4-nitrophenyl propionate + H2O | about 70% activity compared to 4-nitrophenyl butyrate | Bacillus subtilis ECU0554 | 4-nitrophenol + propionate | - |
? | |
| additional information | no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate | Bacillus subtilis | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate | Bacillus subtilis ECU0554 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * about 32000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BsE-NP01 | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus subtilis |
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