Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method, 1.6 A resolution | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
3 * 33000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q3HWU8 | IFO12996 | - |
Pseudomonas putida IFO12996 | Q3HWU8 | IFO12996 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-methyl 2-chloropropionate + H2O | 25% of the activity with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida | ? | - |
? | |
DL-methyl 2-chloropropionate + H2O | as active as with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida | ? | - |
? | |
DL-methyl 2-chloropropionate + H2O | 25% of the activity with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida IFO12996 | ? | - |
? | |
DL-methyl 2-chloropropionate + H2O | as active as with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida IFO12996 | ? | - |
? | |
ethyl DL-beta-acetylthioisobutyrate + H2O | 40% of the activity with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida | ? | - |
? | |
ethyl DL-beta-acetylthioisobutyrate + H2O | 40% of the activity with methyl DL-beta-acetylthioisobutyrate | Pseudomonas putida IFO12996 | ? | - |
? | |
methyl DL-beta-acetylthioisobutyrate + H2O | produces D-beta-acetylthioisobutyrate with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8-10 and an optimum temperature of about 57 to 67°C | Pseudomonas putida | D-beta-acetylthioisobutyrate + methanol | - |
? | |
methyl DL-beta-acetylthioisobutyrate + H2O | produces D-beta-acetylthioisobutyrate with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8-10 and an optimum temperature of about 57 to 67°C | Pseudomonas putida IFO12996 | D-beta-acetylthioisobutyrate + methanol | - |
? | |
additional information | no activity with 2-hydroxyethyl DL-beta-acetylthioisobutyrate | Pseudomonas putida | ? | - |
? | |
additional information | no activity with 2-hydroxyethyl DL-beta-acetylthioisobutyrate | Pseudomonas putida IFO12996 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 33000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | 67 | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Pseudomonas putida |