Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme retains 97% of activity after 60 min in 1 M urea. Not inhibitory: 1% Tween 20, 1% Lubrol | Saccharolobus solfataricus | |
sodium dodecylsulfate | 1%, 60 min at 30°C, 59% residual activity | Saccharolobus solfataricus | |
Triton X-100 | 1%, 60 min at 30°C, 81% residual activity | Saccharolobus solfataricus | |
Tween 80 | 1%, 60 min at 30°C, 76% residual activity | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 40%, 60 min at 30°C, 91% residual activity | Saccharolobus solfataricus |
Acetone | 40%, 60 min at 30°C, 61% residual activity | Saccharolobus solfataricus |
Ethanol | 40%, 60 min at 30°C, 90% residual activity | Saccharolobus solfataricus |
Methanol | 40%, 60 min at 30°C, 86% residual activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus P1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3975 | - |
pH 7.0, 50°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE, MALDI-TOF | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
5 days, 41% residual activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharolobus solfataricus |