Literature summary for 3.1.1.1 extracted from

Kakugawa, S.; Fushinobu, S.; Wakagi, T.; Shoun, H.
Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima (2007), Appl. Microbiol. Biotechnol., 74, 585-591.

Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, with c-terminal His-tag and wizthout the N-terminal hydrophobic region	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	94% residual activity	Thermotoga maritima
diethyl dicarbonate	-	Thermotoga maritima
EDTA	81% residual activity	Thermotoga maritima
EGTA	39% residual activity	Thermotoga maritima
Mn2+	5 mM, 62% of initial activity	Thermotoga maritima
Ni2+	5 mM, 67% of initial activity	Thermotoga maritima
O,O'-bis(2-aminoethyl)ethylenglycol-N,N,N',N'-tetraacetic acid	-	Thermotoga maritima
Phenylmethyl sulfonylfluoride	11% residual activity	Thermotoga maritima
phenylmethylsulfonyl fluoride	-	Thermotoga maritima
Zn2+	5 mM, 41% of initial activity	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00031	-	p-nitrophenyl decanoate	60°C, pH 7.5	Thermotoga maritima
0.0021	-	p-nitrophenyl myristate	60°C, pH 7.5	Thermotoga maritima
0.0047	-	p-nitrophenyl caprylate	60°C, pH 7.5	Thermotoga maritima
0.243	-	p-nitrophenyl butanoate	60°C, pH 7.5	Thermotoga maritima
1.15	-	p-nitrophenyl acetate	60°C, pH 7.5	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	5 mM, 236% of initial activity	Thermotoga maritima
Fe2+	5 mM, 196% of initial activity	Thermotoga maritima
K+	5 mM, 137% of initial activity	Thermotoga maritima
Mg2+	5 mM, 214% of initial activity	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39313	-	x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein	Thermotoga maritima
40000	-	x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein	Thermotoga maritima

Organic Solvent Stability

Organic Solvent	Comment	Organism
dimethyl sulfoxide	5%, 146% of initial activity	Thermotoga maritima
dimethylformamide	5%, 99% of initial activity	Thermotoga maritima
dioxane	5%, 141% of initial activity	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl decanoate + H2O	-	Thermotoga maritima	4-nitrophenol + decanoate	-	?
p-nitrophenyl acetate + H2O	-	Thermotoga maritima	p-nitrophenol + acetate	-	?
p-nitrophenyl butanoate + H2O	-	Thermotoga maritima	p-nitrophenol + butanoate	-	?
p-nitrophenyl caprylate + H2O	-	Thermotoga maritima	p-nitrophenol + caprylate	-	?
p-nitrophenyl decanoate	-	Thermotoga maritima	p-nitrophenol + octanoate	-	?
p-nitrophenyl myristate + H2O	-	Thermotoga maritima	p-nitrophenol + myristate	-	?
p-nitrophenyl palmitate + H2O	50% of the activity with p-nitrophenyl decanoate	Thermotoga maritima	p-nitrophenol + palmitate	-	?
p-nitrophenyl stearate + H2O	50% of the activity with p-nitrophenyl decanoate	Thermotoga maritima	p-nitrophenol + stearate	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE, x * 39313, calculated, His-tagged protein	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	90 min, stable	Thermotoga maritima
80	-	30 min, 50% residual activity	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.9	-	p-nitrophenyl myristate	60°C, pH 7.5	Thermotoga maritima
10.8	-	p-nitrophenyl decanoate	60°C, pH 7.5	Thermotoga maritima
13	-	p-nitrophenyl caprylate	60°C, pH 7.5	Thermotoga maritima
14	-	p-nitrophenyl butanoate	60°C, pH 7.5	Thermotoga maritima
50	-	p-nitrophenyl acetate	60°C, pH 7.5	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	-	Thermotoga maritima

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Release 2023.1 (January 2023)