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Literature summary for 3.1.1.1 extracted from

  • Choi, G.S.; Kim, J.Y.; Kim, J.H.; Ryu, Y.W.; Kim, G.J.
    Construction and characterization of a recombinant esterase with high activity and enantioselectivity to (S)-ketoprofen ethyl ester (2003), Protein Expr. Purif., 29, 85-93.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight activation Pseudomonas fluorescens
Triton X-100 11fold increased activity Pseudomonas fluorescens
Triton X-15 3.3fold increased activity Pseudomonas fluorescens
Triton X-165 9.5fold increased activity Pseudomonas fluorescens
Triton X-305 10.5fold increased activity Pseudomonas fluorescens
Triton X-45 9.4fold increased activity Pseudomonas fluorescens
Tween 20 1.7fold increased activity Pseudomonas fluorescens
Tween 40 6.3fold increased activity Pseudomonas fluorescens
Tween 60 6.2fold increased activity Pseudomonas fluorescens
Tween 80 6.6fold increased activity Pseudomonas fluorescens

Application

Application Comment Organism
biotechnology engineered enzyme can be used to produce the enantiomer (S)-ketoprofen for pharmacological or pharmaceutical use Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
genetic analysis, expression in Escherichia coli as N-terminally His-tagged enzyme Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
2-propanol slight inhibition Pseudomonas fluorescens
acetone slight inhibition Pseudomonas fluorescens
Ag2+ strong inhibition Pseudomonas fluorescens
butanol strong inhibition Pseudomonas fluorescens
ethanol
-
Pseudomonas fluorescens
Fe2+ strong inhibition Pseudomonas fluorescens
Hg2+ strong inhibition Pseudomonas fluorescens
additional information no inhibition by methanol Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000 45000 recombinant enzyme, gel filtration Pseudomonas fluorescens
42000
-
1 * 42000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q8VU79
-
-
Pseudomonas fluorescens KCTC1767 Q8VU79
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77
-
purified recombinant enzyme, substrate 4-nitrophenyl palmitate Pseudomonas fluorescens
387
-
purified recombinant enzyme, substrate 4-nitrophenyl caprylate Pseudomonas fluorescens
2598
-
purified recombinant enzyme, substrate 4-nitrophenyl butyrate Pseudomonas fluorescens
5242
-
purified recombinant enzyme, substrate 4-nitrophenyl acetate Pseudomonas fluorescens
27000
-
purified recombinant enzyme, substrate (S)-ketoprofen ethyl ester Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-ketoprofen ethyl ester + H2O enantioselective activity, best substrate Pseudomonas fluorescens (S)-ketoprofen + ethanol
-
?
(S)-ketoprofen ethyl ester + H2O enantioselective activity, best substrate Pseudomonas fluorescens KCTC1767 (S)-ketoprofen + ethanol
-
?
4-nitrophenyl acetate + H2O
-
Pseudomonas fluorescens 4-nitrophenol + acetate
-
?
4-nitrophenyl acetate + H2O
-
Pseudomonas fluorescens KCTC1767 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Pseudomonas fluorescens 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Pseudomonas fluorescens KCTC1767 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O
-
Pseudomonas fluorescens 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O
-
Pseudomonas fluorescens KCTC1767 4-nitrophenol + caprylate
-
?
4-nitrophenyl palmitate + H2O low activity Pseudomonas fluorescens 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O low activity Pseudomonas fluorescens KCTC1767 4-nitrophenol + palmitate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, recombinant enzyme, SDS-PAGE Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pseudomonas fluorescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 35 stable Pseudomonas fluorescens
40
-
2 h, loss of 50% activity Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
Pseudomonas fluorescens