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Literature summary for 3.1.1.1 extracted from

  • De Simone, G.; Mandrich, L.; Menchise, V.; Giordano, V.; Febbraio, F.; Rossi, M.; Pedone, C.; Manco, G.
    A Substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis (2004), J. Biol. Chem., 279, 6815-6823.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
double-mutant M211S/R215L with bound inhibitor 1-hexadecanesulfonyl chloride, hanging drop vapour diffusion method, equal volumes of protein-inhibitor solution and reservoir solution, the latter containing 2 M ammonium sulfate, 0.1 M Tris buffer, pH 8.5, 2 weeks, X-ray diffraction structure determination and analysis at 2.3 A resolution Alicyclobacillus acidocaldarius

Protein Variants

Protein Variants Comment Organism
M211S/R215L site-directed mutagenesis, increased substrate specificity, altered kinetics compared to the wild-type enzyme Alicyclobacillus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
1-hexadecanesulfonyl chloride irreversible inhibition Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, wild-type and mutant enzyme Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
esterase 2 EST2
-

Reaction

Reaction Comment Organism Reaction ID
a carboxylic ester + H2O = an alcohol + a carboxylate active site structure, substrate binding and reaction mechanism Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl dodecanoate + H2O
-
Alicyclobacillus acidocaldarius 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl hexanoate + H2O
-
Alicyclobacillus acidocaldarius 4-nitrophenol + hexanoate
-
?
additional information substrate specificity of wild-type and mutant enzymes Alicyclobacillus acidocaldarius ?
-
?

Synonyms

Synonyms Comment Organism
EST2
-
Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
wild-type and mutant enzyme Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Alicyclobacillus acidocaldarius

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Alicyclobacillus acidocaldarius
0.00035
-
1-hexadecanesulfonyl chloride wild-type enzyme, pH 7.1, 70°C Alicyclobacillus acidocaldarius
0.00055
-
1-hexadecanesulfonyl chloride mutant M211S/R215L, pH 7.1, 70°C Alicyclobacillus acidocaldarius