Crystallization (Comment) | Organism |
---|---|
double-mutant M211S/R215L with bound inhibitor 1-hexadecanesulfonyl chloride, hanging drop vapour diffusion method, equal volumes of protein-inhibitor solution and reservoir solution, the latter containing 2 M ammonium sulfate, 0.1 M Tris buffer, pH 8.5, 2 weeks, X-ray diffraction structure determination and analysis at 2.3 A resolution | Alicyclobacillus acidocaldarius |
Protein Variants | Comment | Organism |
---|---|---|
M211S/R215L | site-directed mutagenesis, increased substrate specificity, altered kinetics compared to the wild-type enzyme | Alicyclobacillus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hexadecanesulfonyl chloride | irreversible inhibition | Alicyclobacillus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzyme | Alicyclobacillus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | - |
esterase 2 EST2 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a carboxylic ester + H2O = an alcohol + a carboxylate | active site structure, substrate binding and reaction mechanism | Alicyclobacillus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl dodecanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Alicyclobacillus acidocaldarius | 4-nitrophenol + hexanoate | - |
? | |
additional information | substrate specificity of wild-type and mutant enzymes | Alicyclobacillus acidocaldarius | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EST2 | - |
Alicyclobacillus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
wild-type and mutant enzyme | Alicyclobacillus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay at | Alicyclobacillus acidocaldarius |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Alicyclobacillus acidocaldarius | |
0.00035 | - |
1-hexadecanesulfonyl chloride | wild-type enzyme, pH 7.1, 70°C | Alicyclobacillus acidocaldarius | |
0.00055 | - |
1-hexadecanesulfonyl chloride | mutant M211S/R215L, pH 7.1, 70°C | Alicyclobacillus acidocaldarius |