Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, genetic organization, expression in Escherichia coli | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of negative mutant strains, showing reduced activity, by gene replacement for modification of the active site | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | Lactococcus lactis | - |
4-nitrophenol + butyrate | - |
? | |
ethanol + acetate | Lactococcus lactis | ester formation | ethyl acetate + H2O | - |
? | |
ethanol + acetyl-CoA | Lactococcus lactis | ester formation | ethyl acetate + CoA | - |
? | |
ethanol + butyrate | Lactococcus lactis | ester formation | ethyl butanoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | Q8KWI9 | ssp. cremoris, strain CNRZ253, EstA esterase | - |
Lactococcus lactis | Q8KWI9 | ssp. lactis, strain TIL46, EstA esterase | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ester synthesizing activity | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butyrate + H2O | - |
Lactococcus lactis | 4-nitrophenol + butyrate | - |
? | |
ethanol + acetate | ester formation | Lactococcus lactis | ethyl acetate + H2O | - |
? | |
ethanol + acetyl-CoA | ester formation | Lactococcus lactis | ethyl acetate + CoA | - |
? | |
ethanol + butyrate | ester formation | Lactococcus lactis | ethyl butanoate + H2O | - |
? | |
methanethiol + acetyl-CoA | ester formation | Lactococcus lactis | methanethioacetate + coenzyme A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EstA esterase | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ester synthesis | Lactococcus lactis |
8 | - |
assay at | Lactococcus lactis |
8 | - |
assay at, ester hydrolysis | Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 10fold reduced activity at pH 5.0 and 4fold reduced activity at pH 8.0 compared to maximal activity at pH 7.0 | Lactococcus lactis |