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Literature summary for 3.1.1.1 extracted from
- Purification and partial characterization of a novel thermophilic carboxylesterase with high mesophilic specific activity (1995), Enzyme Microb. Technol., 17, 816-825.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| eserine | - |
Geobacillus stearothermophilus |  |
| Hg2+ | HgCl2 | Geobacillus stearothermophilus | |
| p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus | |
| PMSF | - |
Geobacillus stearothermophilus | |
| tosyl-L-Phe | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
4-nitrophenyl laurate | - |
Geobacillus stearothermophilus | |
| 0.011 | - |
4-nitrophenyl caprate | - |
Geobacillus stearothermophilus | |
| 0.012 | - |
4-nitrophenyl octanoate | - |
Geobacillus stearothermophilus | |
| 0.021 | - |
4-nitrophenyl hexanoate | - |
Geobacillus stearothermophilus | |
| 0.031 | - |
4-nitrophenyl pentanoate | - |
Geobacillus stearothermophilus | |
| 0.041 | - |
4-nitrophenyl butyrate | - |
Geobacillus stearothermophilus | |
| 0.05 | - |
4-nitrophenyl propionate | - |
Geobacillus stearothermophilus | |
| 0.055 | - |
4-nitrophenyl acetate | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
gel filtration | Geobacillus stearothermophilus |
| 50000 | - |
1 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.032 | - |
- |
Geobacillus stearothermophilus |
| 1543 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl caprate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + capric acid | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + lauric acid | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl pentanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + pentanoate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + propionate | - |
? | |
| benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
| benzyloxycarbonyl-L-Ala-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-L-Ala + 4-nitrophenol | - |
? | |
| benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester + H2O | - |
Geobacillus stearothermophilus | benzyloxycarbonyl-L-Tyr + 4-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
bovine serum albumin enhances thermostability | Geobacillus stearothermophilus |
| 56 | - |
pH 6.0, stable for 7 days | Geobacillus stearothermophilus |
| 60 | - |
pH 6.0, half-life: 170 h | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | influence of bovine serum albumin on kinetic parameters | Geobacillus stearothermophilus | |
| 555 | - |
4-nitrophenyl laurate | - |
Geobacillus stearothermophilus | |
| 750 | - |
4-nitrophenyl acetate | - |
Geobacillus stearothermophilus | |
| 800 | - |
4-nitrophenyl caprate | - |
Geobacillus stearothermophilus | |
| 1170 | - |
4-nitrophenyl propionate | - |
Geobacillus stearothermophilus | |
| 1250 | - |
4-nitrophenyl octanoate | - |
Geobacillus stearothermophilus | |
| 1380 | - |
4-nitrophenyl butyrate | - |
Geobacillus stearothermophilus | |
| 1550 | - |
4-nitrophenyl hexanoate | - |
Geobacillus stearothermophilus | |
| 15300 | - |
4-nitrophenyl pentanoate | - |
Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
optimum stability | Geobacillus stearothermophilus |
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