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Literature summary for 3.1.1.1 extracted from
- Purification and properties of carboxylesterase B of Escherichia coli (1984), Ann. Microbiol., 135A, 375-387.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Escherichia coli |  |
| Cu2+ | 1 mM, 14% loss of activity | Escherichia coli | |
| DFP | - |
Escherichia coli | |
| Fe2+ | 1 mM, 10% loss of activity | Escherichia coli | |
| Hg2+ | 1 mM, 50% loss of activity | Escherichia coli | |
| Zn2+ | 1 mM, 75% loss of activity | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
1-naphthyl acetate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli HB17 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Escherichia coli | 1-naphthol + acetate | - |
? | |
| 1-naphthyl acetate + H2O | - |
Escherichia coli HB17 | 1-naphthol + acetate | - |
? | |
| 1-naphthyl butyrate + H2O | - |
Escherichia coli | 1-naphthol + butyrate | - |
? | |
| 1-naphthyl butyrate + H2O | - |
Escherichia coli HB17 | 1-naphthol + butyrate | - |
? | |
| 1-naphthyl propionate + H2O | - |
Escherichia coli | 1-naphthol + propionate | - |
? | |
| 1-naphthyl propionate + H2O | - |
Escherichia coli HB17 | 1-naphthol + propionate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Escherichia coli | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Escherichia coli HB17 | 2-naphthol + acetate | - |
? | |
| 2-naphthyl butyrate + H2O | - |
Escherichia coli | 2-naphthol + butyrate | - |
? | |
| 2-naphthyl propionate + H2O | - |
Escherichia coli | 2-naphthol + propionate | - |
? | |
| 2-naphthyl propionate + H2O | - |
Escherichia coli HB17 | 2-naphthol + propionate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxylesterase B | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
10 min, 50% loss of activity | Escherichia coli |
| 70 | - |
10 min, complete loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 30% of maximal activity, pH 8.5: about 40% of maximal cativity | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
30 min, complete inactivation | Escherichia coli |
| 4 | - |
30 min, 80% loss of activity | Escherichia coli |
| 5 | - |
unstable below | Escherichia coli |
| 6 | 10 | 30 min, stable | Escherichia coli |
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