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Literature summary for 2.9.1.1 extracted from

  • Fischer, N.; Paleskava, A.; Gromadski, K.B.; Konevega, A.L.; Wahl, M.C.; Stark, H.; Rodnina, M.V.
    Towards understanding selenocysteine incorporation into bacterial proteins (2007), Biol. Chem., 388, 1061-1067.
    View publication on PubMed
Search for more literature for 2.9.1.1

Cloned(Commentary)

Cloned (Comment) Organism
selA, expression in Escherichia coli strain DH5alpha Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics study of selenocysteine incorporation Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-seryl-tRNASec + selenophosphate Moorella thermoacetica the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview L-selenocysteinyl-tRNASec + phosphate
-
 ?
additional information Moorella thermoacetica SelA interacts with the transcription factor SelB, mutational interaction study, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
 gene selA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-seryl-tRNASec + selenophosphate
-
 Moorella thermoacetica L-selenocysteinyl-tRNASec + phosphate
-
 ?
L-seryl-tRNASec + selenophosphate the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview Moorella thermoacetica L-selenocysteinyl-tRNASec + phosphate
-
 ?
additional information SelA interacts with the transcription factor SelB, mutational interaction study, overview Moorella thermoacetica ?
-
 ?

Subunits

Subunits Comment Organism
decamer the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex Moorella thermoacetica
More formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview Moorella thermoacetica

Synonyms

Synonyms Comment Organism
SelA
-
 Moorella thermoacetica
Selenocysteine synthase
-
 Moorella thermoacetica