Cloned (Comment) | Organism |
---|---|
selA, expression in Escherichia coli strain DH5alpha | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics study of selenocysteine incorporation | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-seryl-tRNASec + selenophosphate | Moorella thermoacetica | the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview | L-selenocysteinyl-tRNASec + phosphate | - |
? | |
additional information | Moorella thermoacetica | SelA interacts with the transcription factor SelB, mutational interaction study, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
gene selA | - |
Purification (Comment) | Organism |
---|---|
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-seryl-tRNASec + selenophosphate | - |
Moorella thermoacetica | L-selenocysteinyl-tRNASec + phosphate | - |
? | |
L-seryl-tRNASec + selenophosphate | the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview | Moorella thermoacetica | L-selenocysteinyl-tRNASec + phosphate | - |
? | |
additional information | SelA interacts with the transcription factor SelB, mutational interaction study, overview | Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex | Moorella thermoacetica |
More | formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview | Moorella thermoacetica |
Synonyms | Comment | Organism |
---|---|---|
SelA | - |
Moorella thermoacetica |
Selenocysteine synthase | - |
Moorella thermoacetica |