Cloned (Comment) | Organism |
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the heterodimeric cytochrome complex SoxXA is produced in Escherichia coli. The soxX and soxA genes, separated by two genes in the sox gene cluster are fused with ribosome binding sites optimal for Escherichia coli and combined to give soxXA in pRD133.27. The cytochrome complex SoxXA is produced in Escherichia coli M15 containing pRD133.27, pREP4 encoding the Lac repressor and plasmid pEC86, carrying essential cytochrome c maturation genes. This cytochrome complex is equally catalytically active with respect to rates and reactivity with different sulfur substrates in the reconstituted sulfur-oxidizing enzyme system using homogeneous Sox-proteins of Paracoccus pantotrophus | Paracoccus pantotrophus |
General Stability | Organism |
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SoxA is unstable when not complexed with SoxX | Paracoccus pantotrophus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c | Paracoccus pantotrophus | the enzyme is involved in the oxidation of reduced inorganic sulfur compounds | [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? | |
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c | Paracoccus pantotrophus | the enzyme is involved in the oxidation of reduced inorganic sulfur compounds | [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | O33434 AND Q9LCV0 | O33434: subunit SoxA, Q9LCV0: subunit SoxX | - |
Purification (Comment) | Organism |
---|---|
the cytochrome complex SoxXA is purified to homogeneity from periplasmic extracts of Escherichia coli M15 | Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c | - |
Paracoccus pantotrophus | [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? | |
[SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c | the enzyme is involved in the oxidation of reduced inorganic sulfur compounds | Paracoccus pantotrophus | [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? | |
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c | - |
Paracoccus pantotrophus | [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? | |
[SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c | the enzyme is involved in the oxidation of reduced inorganic sulfur compounds | Paracoccus pantotrophus | [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 29352 (subunit SoxA) + 1 * 14216 (subunit SoxX), calculated from sequence | Paracoccus pantotrophus |
Synonyms | Comment | Organism |
---|---|---|
complex SoxXA | - |
Paracoccus pantotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one heme is assigned to subunit SoxX and two hemes to subunit SoxA | Paracoccus pantotrophus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the oxidation of reduced inorganic sulfur compounds | Paracoccus pantotrophus |