Literature summary for 2.8.5.1 extracted from

Steiner, E.M.; Boeth, D.; Loessl, P.; Vilaplana, F.; Schnell, R.; Schneider, G.
CysK2 from Mycobacterium tuberculosis is an O-phospho-L-serine-dependent S-sulfocysteine synthase (2014), J. Bacteriol., 196, 3410-3420 .

Search for more literature for 2.8.5.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
R243A	the mutant shows about 10% activity with sulfide compared to the wild type enzyme	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0439	-	thiosulfate	wild type enzyme, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.2137	-	thiosulfate	mutant enzyme R243A, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.4851	-	3-phospho-L-serine	mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis
1.0855	-	3-phospho-L-serine	wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-phospho-L-serine + thiosulfate	Mycobacterium tuberculosis	-	S-sulfo-L-cysteine + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-phospho-L-serine + sulfide	-	Mycobacterium tuberculosis	?	-	?
3-phospho-L-serine + thiosulfate	-	Mycobacterium tuberculosis	S-sulfo-L-cysteine + phosphate	-	?
3-phospho-L-serine + thiosulfate	highest activity	Mycobacterium tuberculosis	S-sulfo-L-cysteine + phosphate	-	?
additional information	the enzyme does not utilize thiocarboxylated CysO as a sulfur donor. O-acetyl-L-serine, L-Asp, L-Val, L-Gln, L-Glu, L-Ser, L-Asn, L-Cys, L-Ser, L-Leu, homocysteine, pyruvate, 2-oxoglutarate, 3-phosphoglycerate, succinate, N-acetylcysteine and diaminopimelic acid are not used as substrate	Mycobacterium tuberculosis	?	-	?

Synonyms

Synonyms	Comment	Organism
CysK2	-	Mycobacterium tuberculosis
O-phosphoserine S-sulfocysteine synthase	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.457	-	thiosulfate	mutant enzyme R243A, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.457	-	3-phospho-L-serine	mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.913	-	thiosulfate	wild type enzyme, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.913	-	3-phospho-L-serine	wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.000083	-	3-phospho-L-serine	wild type enzyme, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.000093	-	3-phospho-L-serine	mutant enzyme R243A, with thiosulfate as cosubstrate, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.0021	-	thiosulfate	mutant enzyme R243A, at pH 9.0 and 22°C	Mycobacterium tuberculosis
0.0208	-	thiosulfate	wild type enzyme, at pH 9.0 and 22°C	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)