Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.4.2 extracted from

  • Villadangos, A.F.; Van Belle, K.; Wahni, K.; Dufe, V.T.; Freitas, S.; Nur, H.; De Galan, S.; Gil, J.A.; Collet, J.F.; Mateos, L.M.; Messens, J.
    Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms (2011), Mol. Microbiol., 82, 998-1014.
    View publication on PubMed
Search for more literature for 2.8.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform ArsC2, hanging drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5 Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
0.142
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arsenate + mycothiol Corynebacterium glutamicum
-
 arseno-mycothiol + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum P0DKS7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + mycothiol
-
 Corynebacterium glutamicum arseno-mycothiol + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
ArsC1 isoform Corynebacterium glutamicum
ArsC2 isoform Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
0.53
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
3.8
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum