Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-bromopropane sulfonate | BPS, forms an alkyl-Ni(III) species with MCRred1is that elicits the MCRPS EPR signal; irreversible inhibition | Methanothermobacter marburgensis | |
4-bromobutane sulfonate | when reacted with 4-bromobutyrate, MCRred1 forms the alkyl-Ni(III) MCRXA state and then self-reactivation to regenerate the Ni(I) MCRred1 state and a bromocarboxy ester | Methanothermobacter marburgensis | |
bromopropionate | - |
Methanothermobacter marburgensis | |
additional information | inhibition mechanism, brominated carboxylic acids, with carbon chain lengths of 4-16, all give rise to an alkyl-Ni intermediate with an EPR signal similar to that of the MCRPS species, brominated carboxylic acids mimic the interactions of BPS and methyl-SCoM at the MCR active site, overview | Methanothermobacter marburgensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of MCRred1 with brominated acids, overview | Methanothermobacter marburgensis | |
additional information | - |
additional information | transient kinetics study, overview | Methanothermobacter marburgensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3-S-CoM + HS-CoB | Methanothermobacter marburgensis | i.e. methyl-coenzyme M + coenzyme B | CoM-S-S-CoB + methane | - |
? | |
methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) | Methanothermobacter marburgensis | i.e. methyl-SCoM | methane + CoB-S-S-CoM | a the mixed disulfide | ? | |
additional information | Methanothermobacter marburgensis | MCR catalyzes the final step in the biological synthesis of methane. Using coenzyme B, CoBSH, as the two-electron donor, MCR reduces methyl-coenzyme M, methyl-SCoM, to methane and the mixed disulfide, CoB-S-S-CoM. MCR contains coenzyme F430, an essential redox-active nickel tetrahydrocorphin, at its active site. The active form of MCR, MCRred1, contains Ni(I)-F430 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter marburgensis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methyl-CoM + CoB = CoM-S-S-CoB + methane | mechanism, nucleophilic attack of Ni(I)-MCRred1 on the methyl group of methyl-SCoM generates a methyl-Ni intermediate | Methanothermobacter marburgensis | |
methyl-CoM + CoB = CoM-S-S-CoB + methane | Two reaction mechanisms for methane formation distinguishable by the first step of catalysis, overview | Methanothermobacter marburgensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3-S-CoM + HS-CoB | i.e. methyl-coenzyme M + coenzyme B | Methanothermobacter marburgensis | CoM-S-S-CoB + methane | - |
? | |
CH3-S-CoM + HS-CoB | i.e. methyl-coenzyme M + coenzyme B, selectivity of the MCR reaction toward nucleophilic attack by Ni(I) | Methanothermobacter marburgensis | CoM-S-S-CoB + methane | - |
? | |
methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) | i.e. methyl-SCoM | Methanothermobacter marburgensis | methane + CoM-S-S-CoB | a the mixed disulfide | ? | |
methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) | i.e. methyl-SCoM | Methanothermobacter marburgensis | methane + CoB-S-S-CoM | a the mixed disulfide | ? | |
methylmercaptopropionate + HS-CoB | is about 110fold less reactive than the natural substrate methyl-SCoM | Methanothermobacter marburgensis | ? | - |
? | |
additional information | the enzyme performs reactions with brominated acid, 4-bromobutyric acid to 16-bromohexadecanoic acid, analogously to the reaction of MCRred1, the active Ni(I)-F430 containing enzyme form, to generate a methyl-Ni(III) intermediate in methane formation with the natural substrate, methyl-SCoM, substrate specificity and acivities, overview | Methanothermobacter marburgensis | ? | - |
? | |
additional information | MCR catalyzes the final step in the biological synthesis of methane. Using coenzyme B, CoBSH, as the two-electron donor, MCR reduces methyl-coenzyme M, methyl-SCoM, to methane and the mixed disulfide, CoB-S-S-CoM. MCR contains coenzyme F430, an essential redox-active nickel tetrahydrocorphin, at its active site. The active form of MCR, MCRred1, contains Ni(I)-F430 | Methanothermobacter marburgensis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCR | - |
Methanothermobacter marburgensis |
methyl-coenzyme M reductase | - |
Methanothermobacter marburgensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methanothermobacter marburgensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Methanothermobacter marburgensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F430 | an essential redox-active nickel tetrahydrocorphin, bound at the active site, the active form of MCR, MCRred1, contains Ni(I)-F430 | Methanothermobacter marburgensis | |
F-430 | with Ni(I) oxidation state, selectivity of the MCR reaction toward nucleophilic attack by Ni(I) | Methanothermobacter marburgensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
3-bromopropane sulfonate | - |
Methanothermobacter marburgensis |