Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ti(III) citrate | prevents enzyme inactivation by oxygen | Methanothrix soehngenii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
coenzyme B | - |
Methanothrix soehngenii | |
2 | - |
methyl-coenzyme M | - |
Methanothrix soehngenii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30400 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
41700 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
63900 | - |
1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
280000 | - |
gel filtration | Methanothrix soehngenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothrix soehngenii | - |
DSM 2139 | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose, Phenyl-Superose HR 5/5, Superose 6 HR 10/30 column | Methanothrix soehngenii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.051 | - |
- |
Methanothrix soehngenii |
Storage Stability | Organism |
---|---|
-20°C, stored under N2 atmosphere, 50 mM anaerobic Tris-HCl, pH 7.6, 150 mM NaCl, 1 mM Ti(III) citrate | Methanothrix soehngenii |
4°C | Methanothrix soehngenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-coenzyme M + coenzyme B | - |
Methanothrix soehngenii | methane + CoM-S-S-CoB | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 63900, 1 * 41700, 1 * 30400, SDS-PAGE | Methanothrix soehngenii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanothrix soehngenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
60 nmol of methane are formed per minute per mg protein | Methanothrix soehngenii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
F-430 | contains two tightly bound molecules of coenzyme F-430 | Methanothrix soehngenii |