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Literature summary for 2.8.4.1 extracted from

  • Jablonski, P.E.; Ferry, J.G.
    Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila (1991), J. Bacteriol., 173, 2481-2487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.4.1

Activating Compound

Activating Compound Comment Organism Structure
ATP stimulates the reactivation Methanosarcina thermophila
reduced ferredoxin reactivation after purification Methanosarcina thermophila
Ti(III) citrate reactivation after isolation Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 methyl coenzyme M
-
 Methanosarcina thermophila
0.059
-
 coenzyme B
-
 Methanosarcina thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information no activity found in membrane fraction Methanosarcina thermophila
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
42000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
69000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
132000
-
 nondenaturing slab gel electrophoresis Methanosarcina thermophila
141000
-
 gel filtration Methanosarcina thermophila
144000
-
-
 Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl coenzyme M + coenzyme B Methanosarcina thermophila
-
 methane + CoM-S-S-CoB
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
 TM-1, acetate grown
-

Purification (Commentary)

Purification (Comment) Organism
sucrose density gradient cetrifugation, Q-Sepharose, Phenyl-Superose 5/5 column, anaerobic Methanosarcina thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.224
-
-
 Methanosarcina thermophila

Storage Stability

Storage Stability Organism
4°C, 11 h, fully active Methanosarcina thermophila
liquid N2, 50 mM potassium phosphate buffer, pH 7.0, 1 M potassium acetate, 10 mM 2-mercaptoethanol, 10% w/v glycerol Methanosarcina thermophila
three freeze-thaw cycles, fully active Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl coenzyme M + coenzyme B
-
 Methanosarcina thermophila methane + CoM-S-S-CoB
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Methanosarcina thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 11 h, fully active Methanosarcina thermophila
23
-
 N2 atmosphere, 2 h, 28% loss of original activity Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Methanosarcina thermophila

Cofactor

Cofactor Comment Organism Structure
F-430 1 mol coenzyme F-430/mol enzyme Methanosarcina thermophila