BRENDA - Enzyme Database
show all sequences of 2.8.3.9

Butyryl-CoA:acetoacetate CoA-transferase from a lysine-fermenting Clostridium

Barker, H.A.; Jemg, I.M.; Neff, N.; Robertson, J.M.; Tam, F.K.; Hosaka, S.; J. Biol. Chem. 253, 1219-1225 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Tris-HCl
activation at 0.25 M, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
General Stability
General Stability
Organism
purified enzyme is very stable, considerably less stable in Tris-HCl than in phosphate buffer
Clostridium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
acetoacetyl-CoA
pH 7.0, 25°C, cosubstrate crotonate
Clostridium sp.
0.023
-
butyryl-CoA
pH 7.0, 25°C, cosubstrate crotonate
Clostridium sp.
0.034
-
butyryl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.044
-
butyryl-CoA
pH 6.3, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.054
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 1.0 mM, 20 mM MgCl2
Clostridium sp.
0.073
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 2.0 mM, 20 mM MgCl2
Clostridium sp.
0.083
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 3.0 mM, 20 mM MgCl2
Clostridium sp.
0.135
-
acetyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.167
-
crotonyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.205
-
propionyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.8
-
Butanoate
pH 7.5, 25°C
Clostridium sp.
1.1
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.18 mM
Clostridium sp.
1.4
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.23 mM
Clostridium sp.
1.8
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.37 mM
Clostridium sp.
2
-
crotonate
pH 7.5, 25°C
Clostridium sp.
3.2
-
propionate
pH 7.5, 25°C
Clostridium sp.
7
-
pentanoate
pH 7.5, 25°C
Clostridium sp.
14
-
Isobutyrate
pH 7.5, 25°C
Clostridium sp.
17
-
Vinylacetate
pH 7.5, 25°C
Clostridium sp.
21
-
DL-2-Hydroxypentanoate
pH 7.5, 25°C
Clostridium sp.
21
-
Isopentanoate
pH 7.5, 25°C
Clostridium sp.
24
-
acetate
pH 7.5, 25°C
Clostridium sp.
29
-
3-Mercaptopropionate
pH 7.5, 25°C
Clostridium sp.
29
-
Monochloroacetate
pH 7.5, 25°C
Clostridium sp.
33
-
4-hydroxybuyrate
pH 7.5, 25°C
Clostridium sp.
40
-
Acrylate
pH 7.5, 25°C
Clostridium sp.
42
-
3-Methylvinylacetate
pH 7.5, 25°C
Clostridium sp.
44
-
DL-3-hydroxybutyrate
pH 7.5, 25°C
Clostridium sp.
49
-
DL-2-hydroxybuyrate
pH 7.5, 25°C
Clostridium sp.
250
-
3-hydroxypropionate
pH 7.5, 25°C
Clostridium sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation at 6 mM CaCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
K+
activation at 0.08 M KCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Li+
activation at 0.2 M LiCl, inhibitions at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Mg2+
activation at 12 mM MgCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Mn2+
activation at 1.2 mM MnCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Na+
activation at 0.17 M NaCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
NH4+
activation at 0.08 M NH4Cl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
25100
-
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
90000
-
gel electrophoresis
Clostridium sp.
108000
-
gel filtration
Clostridium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoate + acetoacetyl-CoA
Acetoanaerobium sticklandii
-
butanoyl-CoA + acetoacetate
-
Acetoanaerobium sticklandii
r
butanoate + acetoacetyl-CoA
Clostridium sp.
lysine degradation pathway
butanoyl-CoA + acetoacetate
-
Clostridium sp.
r
butanoate + acetoacetyl-CoA
Clostridium sp. SB4
lysine degradation pathway
butanoyl-CoA + acetoacetate
-
Clostridium sp. SB4
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acetoanaerobium sticklandii
-
-
-
Clostridium sp.
-
SB4
-
Clostridium sp. SB4
-
SB4
-
Purification (Commentary)
Commentary
Organism
-
Clostridium sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
329
-
-
Clostridium sp.
Storage Stability
Storage Stability
Organism
-15°C 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of 12 days when thawed and refrozen 5 times
Clostridium sp.
-15°C, 3.1 mg protein/ml, 20 mM potassium phosphate, pH 7.0, retains full activity during storage for 1 year
Clostridium sp.
4°C, 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of about 7 days
Clostridium sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropanoate + acetoacetyl-CoA
reaction at 8.6% the rate of butyrate
643838
Clostridium sp.
3-hydroxypropanoyl-CoA + acetoacetate
-
-
-
r
3-mercaptopropionate + acetoacetyl-CoA
reaction at 71% the rate of butyrate
643838
Clostridium sp.
3-mercaptopropionyl-CoA + acetoacetate
-
-
-
r
3-methylvinylacetate + acetoacetyl-CoA
reaction at 44% the rate of butyrate
643838
Clostridium sp.
3-methylvinylacetyl-CoA + acetoacetate
-
-
-
r
4-hydroxybutyrate + acetoacetyl-CoA
reaction at 14% the rate of butyrate
643838
Clostridium sp.
4-hydroxybutanoyl-CoA + acetoacetate
-
-
-
r
acetate + acetoacetyl-CoA
-
643838
Clostridium sp.
acetyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
acetate + acetoacetyl-CoA
reaction at 24% the rate of butyrate
643838
Clostridium sp.
acetyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
acetate + acetoacetyl-CoA
-
643838
Clostridium sp. SB4
acetyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
acetate + acetoacetyl-CoA
reaction at 24% the rate of butyrate
643838
Clostridium sp. SB4
acetyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
acrylate + acetoacetyl-CoA
reaction at 10% the rate of butyrate
643838
Clostridium sp.
acrylyl-CoA + acetoacetate
-
-
-
r
butanoate + acetoacetyl-CoA
-
643838
Acetoanaerobium sticklandii
butanoyl-CoA + acetoacetate
-
643838
Acetoanaerobium sticklandii
r
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp.
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
butanoate + acetoacetyl-CoA
lysine degradation pathway
643838
Clostridium sp.
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp. SB4
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
butanoate + acetoacetyl-CoA
lysine degradation pathway
643838
Clostridium sp. SB4
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
butanoyl-CoA + 2-methylacetoacetate
used 12% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 2-methylacetoacetyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + 3-oxohexanoate
used 21% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 3-oxohexanoyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + 3-oxopentanoate
used 60% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 3-oxopentanoyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp.
r
crotonate + acetoacetyl-CoA
reaction at 16% the rate of butyrate
643838
Clostridium sp.
crotonyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
DL-2-hydroxybutyrate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
2-hydroxybutyryl-CoA + acetoacetate
-
-
-
r
DL-2-hydroxypentanoate + acetoacetyl-CoA
reaction at 7.9% the rate of butyrate
643838
Clostridium sp.
2-hydroxypentanoyl-CoA + acetoacetate
-
-
-
r
DL-3-hydroxybutyrate + acetoacetyl-CoA
reaction at 30% the rate of butyrate
643838
Clostridium sp.
3-hydroxybutyryl-CoA + acetoacetate
-
-
-
r
isobutyrate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
isobutyryl-CoA + acetoacetate
-
-
-
r
isopentanoate + acetoacetyl-CoA
reaction at 8.3% the rate of butyrate
643838
Clostridium sp.
isopentanoyl-CoA + acetoacetate
-
-
-
r
monochloroacetate + acetoacetyl-CoA
reaction at 5.4% the rate of butyrate
643838
Clostridium sp.
monochloroacetyl-CoA + acetoacetate
-
-
-
r
additional information
monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive
643838
Clostridium sp.
?
-
-
-
-
additional information
monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive
643838
Clostridium sp. SB4
?
-
-
-
-
pentanoate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
pentanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
propionate + acetoacetyl-CoA
reaction at 21% the rate of butyrate
643838
Clostridium sp.
propionyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
vinylacetate + acetoacetyl-CoA
best substrate
643838
Clostridium sp.
vinylacetyl-CoA + acetoacetate
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
1 h, in potassium phosphate buffer, pH 6.5-7.0, most stable, about 20% loss of activity at pH 6.0 and 8.0
Clostridium sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8
-
Clostridium sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
9
maximally active from pH 6.6 to pH 7.9
Clostridium sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
7
1 h, at 30°C, most stable, about 20% loss of activity at pH 6.0 and 8.0
Clostridium sp.
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Tris-HCl
activation at 0.25 M, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
General Stability (protein specific)
General Stability
Organism
purified enzyme is very stable, considerably less stable in Tris-HCl than in phosphate buffer
Clostridium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
acetoacetyl-CoA
pH 7.0, 25°C, cosubstrate crotonate
Clostridium sp.
0.023
-
butyryl-CoA
pH 7.0, 25°C, cosubstrate crotonate
Clostridium sp.
0.034
-
butyryl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.044
-
butyryl-CoA
pH 6.3, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.054
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 1.0 mM, 20 mM MgCl2
Clostridium sp.
0.073
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 2.0 mM, 20 mM MgCl2
Clostridium sp.
0.083
-
butyryl-CoA
pH 8.1, 25°C, cosubstrate acetoacetate 3.0 mM, 20 mM MgCl2
Clostridium sp.
0.135
-
acetyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.167
-
crotonyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.205
-
propionyl-CoA
pH 7.0, 25°C, cosubstrate acetoacetate
Clostridium sp.
0.8
-
Butanoate
pH 7.5, 25°C
Clostridium sp.
1.1
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.18 mM
Clostridium sp.
1.4
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.23 mM
Clostridium sp.
1.8
-
acetoacetate
pH 7.5, 25°C, butyryl-CoA level of 0.37 mM
Clostridium sp.
2
-
crotonate
pH 7.5, 25°C
Clostridium sp.
3.2
-
propionate
pH 7.5, 25°C
Clostridium sp.
7
-
pentanoate
pH 7.5, 25°C
Clostridium sp.
14
-
Isobutyrate
pH 7.5, 25°C
Clostridium sp.
17
-
Vinylacetate
pH 7.5, 25°C
Clostridium sp.
21
-
DL-2-Hydroxypentanoate
pH 7.5, 25°C
Clostridium sp.
21
-
Isopentanoate
pH 7.5, 25°C
Clostridium sp.
24
-
acetate
pH 7.5, 25°C
Clostridium sp.
29
-
3-Mercaptopropionate
pH 7.5, 25°C
Clostridium sp.
29
-
Monochloroacetate
pH 7.5, 25°C
Clostridium sp.
33
-
4-hydroxybuyrate
pH 7.5, 25°C
Clostridium sp.
40
-
Acrylate
pH 7.5, 25°C
Clostridium sp.
42
-
3-Methylvinylacetate
pH 7.5, 25°C
Clostridium sp.
44
-
DL-3-hydroxybutyrate
pH 7.5, 25°C
Clostridium sp.
49
-
DL-2-hydroxybuyrate
pH 7.5, 25°C
Clostridium sp.
250
-
3-hydroxypropionate
pH 7.5, 25°C
Clostridium sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation at 6 mM CaCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
K+
activation at 0.08 M KCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Li+
activation at 0.2 M LiCl, inhibitions at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Mg2+
activation at 12 mM MgCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Mn2+
activation at 1.2 mM MnCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Na+
activation at 0.17 M NaCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
NH4+
activation at 0.08 M NH4Cl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate
Clostridium sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
25100
-
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
90000
-
gel electrophoresis
Clostridium sp.
108000
-
gel filtration
Clostridium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butanoate + acetoacetyl-CoA
Acetoanaerobium sticklandii
-
butanoyl-CoA + acetoacetate
-
Acetoanaerobium sticklandii
r
butanoate + acetoacetyl-CoA
Clostridium sp.
lysine degradation pathway
butanoyl-CoA + acetoacetate
-
Clostridium sp.
r
butanoate + acetoacetyl-CoA
Clostridium sp. SB4
lysine degradation pathway
butanoyl-CoA + acetoacetate
-
Clostridium sp. SB4
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
329
-
-
Clostridium sp.
Storage Stability (protein specific)
Storage Stability
Organism
-15°C 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of 12 days when thawed and refrozen 5 times
Clostridium sp.
-15°C, 3.1 mg protein/ml, 20 mM potassium phosphate, pH 7.0, retains full activity during storage for 1 year
Clostridium sp.
4°C, 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of about 7 days
Clostridium sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropanoate + acetoacetyl-CoA
reaction at 8.6% the rate of butyrate
643838
Clostridium sp.
3-hydroxypropanoyl-CoA + acetoacetate
-
-
-
r
3-mercaptopropionate + acetoacetyl-CoA
reaction at 71% the rate of butyrate
643838
Clostridium sp.
3-mercaptopropionyl-CoA + acetoacetate
-
-
-
r
3-methylvinylacetate + acetoacetyl-CoA
reaction at 44% the rate of butyrate
643838
Clostridium sp.
3-methylvinylacetyl-CoA + acetoacetate
-
-
-
r
4-hydroxybutyrate + acetoacetyl-CoA
reaction at 14% the rate of butyrate
643838
Clostridium sp.
4-hydroxybutanoyl-CoA + acetoacetate
-
-
-
r
acetate + acetoacetyl-CoA
-
643838
Clostridium sp.
acetyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
acetate + acetoacetyl-CoA
reaction at 24% the rate of butyrate
643838
Clostridium sp.
acetyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
acetate + acetoacetyl-CoA
-
643838
Clostridium sp. SB4
acetyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
acetate + acetoacetyl-CoA
reaction at 24% the rate of butyrate
643838
Clostridium sp. SB4
acetyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
acrylate + acetoacetyl-CoA
reaction at 10% the rate of butyrate
643838
Clostridium sp.
acrylyl-CoA + acetoacetate
-
-
-
r
butanoate + acetoacetyl-CoA
-
643838
Acetoanaerobium sticklandii
butanoyl-CoA + acetoacetate
-
643838
Acetoanaerobium sticklandii
r
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp.
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
butanoate + acetoacetyl-CoA
lysine degradation pathway
643838
Clostridium sp.
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp. SB4
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
butanoate + acetoacetyl-CoA
lysine degradation pathway
643838
Clostridium sp. SB4
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp. SB4
r
butanoyl-CoA + 2-methylacetoacetate
used 12% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 2-methylacetoacetyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + 3-oxohexanoate
used 21% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 3-oxohexanoyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + 3-oxopentanoate
used 60% as fast as acetoacetate
643838
Clostridium sp.
butanoate + 3-oxopentanoyl-CoA
-
643838
Clostridium sp.
r
butanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
butanoate + acetoacetyl-CoA
-
643838
Clostridium sp.
r
crotonate + acetoacetyl-CoA
reaction at 16% the rate of butyrate
643838
Clostridium sp.
crotonyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
DL-2-hydroxybutyrate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
2-hydroxybutyryl-CoA + acetoacetate
-
-
-
r
DL-2-hydroxypentanoate + acetoacetyl-CoA
reaction at 7.9% the rate of butyrate
643838
Clostridium sp.
2-hydroxypentanoyl-CoA + acetoacetate
-
-
-
r
DL-3-hydroxybutyrate + acetoacetyl-CoA
reaction at 30% the rate of butyrate
643838
Clostridium sp.
3-hydroxybutyryl-CoA + acetoacetate
-
-
-
r
isobutyrate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
isobutyryl-CoA + acetoacetate
-
-
-
r
isopentanoate + acetoacetyl-CoA
reaction at 8.3% the rate of butyrate
643838
Clostridium sp.
isopentanoyl-CoA + acetoacetate
-
-
-
r
monochloroacetate + acetoacetyl-CoA
reaction at 5.4% the rate of butyrate
643838
Clostridium sp.
monochloroacetyl-CoA + acetoacetate
-
-
-
r
additional information
monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive
643838
Clostridium sp.
?
-
-
-
-
additional information
monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive
643838
Clostridium sp. SB4
?
-
-
-
-
pentanoate + acetoacetyl-CoA
reaction at 19% the rate of butyrate
643838
Clostridium sp.
pentanoyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
propionate + acetoacetyl-CoA
reaction at 21% the rate of butyrate
643838
Clostridium sp.
propionyl-CoA + acetoacetate
-
643838
Clostridium sp.
r
vinylacetate + acetoacetyl-CoA
best substrate
643838
Clostridium sp.
vinylacetyl-CoA + acetoacetate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE
Clostridium sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
1 h, in potassium phosphate buffer, pH 6.5-7.0, most stable, about 20% loss of activity at pH 6.0 and 8.0
Clostridium sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8
-
Clostridium sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
9
maximally active from pH 6.6 to pH 7.9
Clostridium sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
7
1 h, at 30°C, most stable, about 20% loss of activity at pH 6.0 and 8.0
Clostridium sp.
Other publictions for EC 2.8.3.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737542
Yu
Metabolic engineering of Clost ...
Clostridium tyrobutyricum
Appl. Microbiol. Biotechnol.
99
4917-4930
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643839
Emmanuel
-
Butyrate:acetoacetyl-CoA trans ...
Bos taurus
Can. J. Anim. Sci.
63
355-360
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
643838
Barker
Butyryl-CoA:acetoacetate CoA-t ...
Acetoanaerobium sticklandii, Clostridium sp., Clostridium sp. SB4
J. Biol. Chem.
253
1219-1225
1978
1
-
-
-
-
1
-
30
-
7
4
3
-
14
-
-
1
-
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
30
-
7
4
3
-
-
-
1
-
-
1
3
30
1
-
-
1
-
1
1
1
-
-
-
-
-
-
-
643836
Sramek
Escherichia coli coenzyme A-tr ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
27-35
1975
-
-
-
-
-
-
2
2
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643837
Sramek
Purification and properties of ...
Escherichia coli, Escherichia coli C22
Arch. Biochem. Biophys.
171
14-26
1975
-
-
-
-
-
-
3
2
2
-
5
2
-
3
-
1
1
-
-
-
2
-
17
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
2
2
-
5
2
-
-
1
1
-
-
2
-
17
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-